Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures

TOPINKOVÁ, Jana, Libuše TRNKOVÁ and Josef HAVEL. Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures. In Chemické listy. Praha: Česká společnost chemická, 2002, p. 482. ISBN 0009-2770.

Basic information

• Original name: Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures
• Authors: TOPINKOVÁ, Jana (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and Josef HAVEL (203 Czech Republic).
• Edition: Praha, Chemické listy, p. 482-482, 2002.
• Publisher: Česká společnost chemická

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/02:00006278
• Organization unit: Faculty of Science
• ISBN: 0009-2770
• Keywords in English: adenine;cytosine;UV-Vis spectra;artificial neural networks;TRAJAN 6;chemometrics
• Tags: adenine, artificial neural networks, chemometrics, cytosine, TRAJAN 6, UV-Vis spectra

Abstract

Recently we have demonstrated advantages of artificial neural networks (ANNs) for quantification of nucleic acid bases mixtures from overlapping voltammetric signals. In this work ANNs were applied to analyze adenine-cytosine mixtures from UV-Vis spectra. Spectra of these compounds are highly similar (lambda max 261 and 270nm, respectively) and common spectral approaches of analysis are failing. The optimal ANN architecture, able to model the system, was established. Software of ANNs method was from TRAJAN 6 program. Using the suitable experimental design with soft ANN modeling adenine - cytosine mixtures can be quantified with an excellent precision (less than 1%). Generally, ANNs open many possibilities for the analysis of mixtures of biologically important substances with very overlapped signals, even without the explicit knowledge of their behavior.

Links

• GA203/02/0422, research and development project: Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

• GV204/97/K084, research and development project: Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

• IAA1163201, research and development project: Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

• MSM 143100011, plan (intention): Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles