Další formáty:
BibTeX
LaTeX
RIS
@article{404708,
author = {Dubový, Petr and Klusáková, Ilona and Svíženská, Ivana},
article_location = {Heidelberg},
article_number = {6},
keywords = {extracellular; matrix; regeneration},
language = {eng},
issn = {0948-6143},
journal = {Histochemistry and Cell Biology},
title = {A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots},
volume = {117},
year = {2002}
}
TY - JOUR
ID - 404708
AU - Dubový, Petr - Klusáková, Ilona - Svíženská, Ivana
PY - 2002
TI - A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots
JF - Histochemistry and Cell Biology
VL - 117
IS - 6
SP - 473
EP - 473
PB - Springer
SN - 09486143
KW - extracellular
KW - matrix
KW - regeneration
N2 - The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
ER -
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2002, roč.~117, č.~6, s.~473-480. ISSN~0948-6143.
|
