DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. Histochemistry and Cell Biology. Heidelberg: Springer, 2002, vol. 117, No 6, p. 473-480. ISSN 0948-6143.
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Basic information
Original name A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots
Name in Czech Kvantitativní imunohistochemické studium endoneuria dorzálních a ventrálních kořenů spinálních nervů
Authors DUBOVÝ, Petr (203 Czech Republic, guarantor), Ilona KLUSÁKOVÁ (203 Czech Republic) and Ivana SVÍŽENSKÁ (203 Czech Republic).
Edition Histochemistry and Cell Biology, Heidelberg, Springer, 2002, 0948-6143.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30000 3. Medical and Health Sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.054
RIV identification code RIV/00216224:14110/02:00006287
Organization unit Faculty of Medicine
UT WoS 000176933000002
Keywords in English extracellular; matrix; regeneration
Tags extracellular, matrix, Regeneration
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Dubový, CSc., učo 698. Changed: 17/6/2009 10:45.
Abstract
The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
Abstract (in Czech)
Endoneuriální ECM podél nervových vláken ve dorzálních a ventrálních kořenech spinálních nervů mají rozdílné molekulární složení.
Links
GA309/00/0407, research and development projectName: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů
Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves
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