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@article{405042,
author = {Machát, Jiří and Kanický, Viktor and Otruba, Vítězslav},
article_location = {Heidelberg},
article_number = {4},
keywords = {Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical Chemistry},
title = {Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization},
volume = {372},
year = {2002}
}
TY - JOUR
ID - 405042
AU - Machát, Jiří - Kanický, Viktor - Otruba, Vítězslav
PY - 2002
TI - Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization
JF - Analytical and Bioanalytical Chemistry
VL - 372
IS - 4
SP - 576
EP - 576
PB - Springer
SN - 16182642
KW - Selenium speciation
KW - Spectrometry
KW - ICP-AES
KW - Pneumatic nebulization
N2 - The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181.
ER -
MACHÁT, Jiří, Viktor KANICKÝ and Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: Springer, vol.~372, No~4, p.~576-581. ISSN~1618-2642. 2002.
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