MACHÁT, Jiří, Viktor KANICKÝ and Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. Analytical and Bioanalytical Chemistry. Heidelberg: Springer, 2002, vol. 372, No 4, p. 576-581. ISSN 1618-2642. |
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@article{405042, author = {Machát, Jiří and Kanický, Viktor and Otruba, Vítězslav}, article_location = {Heidelberg}, article_number = {4}, keywords = {Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization}, language = {eng}, issn = {1618-2642}, journal = {Analytical and Bioanalytical Chemistry}, title = {Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization}, volume = {372}, year = {2002} }
TY - JOUR ID - 405042 AU - Machát, Jiří - Kanický, Viktor - Otruba, Vítězslav PY - 2002 TI - Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization JF - Analytical and Bioanalytical Chemistry VL - 372 IS - 4 SP - 576 EP - 576 PB - Springer SN - 16182642 KW - Selenium speciation KW - Spectrometry KW - ICP-AES KW - Pneumatic nebulization N2 - The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181. ER -
MACHÁT, Jiří, Viktor KANICKÝ and Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: Springer, 2002, vol.~372, No~4, p.~576-581. ISSN~1618-2642.
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