| MAZOCH, Jiří and Igor KUČERA. Detection, with a pH indicator, of bacterial mutants unable to denitrify. Journal of Microbiological Methods. Amsterdam: Elsevier Science B.V., 2002, vol. 51, No 1, p. 105-109. ISSN 0167-7012. |
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@article{405241,
author = {Mazoch, Jiří and Kučera, Igor},
article_location = {Amsterdam},
article_number = {1},
keywords = {Denitrification; pH indicator; Transposon mutagenesis},
language = {eng},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
title = {Detection, with a pH indicator, of bacterial mutants unable to denitrify},
volume = {51},
year = {2002}
}
TY - JOUR
ID - 405241
AU - Mazoch, Jiří - Kučera, Igor
PY - 2002
TI - Detection, with a pH indicator, of bacterial mutants unable to denitrify
JF - Journal of Microbiological Methods
VL - 51
IS - 1
SP - 105
EP - 105
PB - Elsevier Science B.V.
SN - 01677012
KW - Denitrification
KW - pH indicator
KW - Transposon mutagenesis
N2 - In order to facilitate isolation of mutants with alterations in the denitrification pathway, a new screening procedure using phenol red incorporated into agar overlay has been defined. Alkalinization in the neighbourhood of denitrifying colonies respiring nitrate or nitrite gives rise to a red circular halo. Antimycin blocked these colour changes, which suggests their association with the periplasmic reduction of nitrite. Inhibition of nitrous oxide reductase by acetylene had no significant effect on alkalinization elicited by nitrate or nitrite. Several mutants negative by the phenol red staining test were generated by transposon Tn5 mutagenesis of Paracoccus denitrificans. All these mutants were defective in the activities of nitrite and nitric oxide reductases while the other denitrification activities were present at the wild-type level.
ER -
MAZOCH, Jiří and Igor KUČERA. Detection, with a pH indicator, of bacterial mutants unable to denitrify. \textit{Journal of Microbiological Methods}. Amsterdam: Elsevier Science B.V., 2002, vol.~51, No~1, p.~105-109. ISSN~0167-7012.
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