BENEŠ, Petr, G. KOELSCH, B. DVOŘÁK, M. FUSEK and V. VETVICKA. Detection of procathepsin D in rat milk. Comparative Biochemistry and Physiology B - Biochemistry & Molecular Biology. ELSEVIER SCIENCE, 2002, vol. 2002, No 133, p. 113-118. ISSN 1096-4959.
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Basic information
Original name Detection of procathepsin D in rat milk
Name in Czech Detekce prokatepsinu D v krysím mléku.
Authors BENEŠ, Petr (203 Czech Republic, guarantor), G. KOELSCH (840 United States of America), B. DVOŘÁK (203 Czech Republic), M. FUSEK (203 Czech Republic) and V. VETVICKA (203 Czech Republic).
Edition Comparative Biochemistry and Physiology B - Biochemistry & Molecular Biology, ELSEVIER SCIENCE, 2002, 1096-4959.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.195
Organization unit Faculty of Science
Keywords in English Aspartic protease; Cathepsin D; Procathepsin D; Rat milk; Zymogen
Tags Aspartic protease, cathepsin D, procathepsin D, Rat milk, Zymogen
Tags International impact, Reviewed
Changed by Changed by: doc. Mgr. Petr Beneš, Ph.D., učo 2082. Changed: 2/10/2008 14:06.
Abstract
The presence of procathepsin D, a zymogen of the soluble lysosomal aspartic proteinase cathepsin D, was detected in rat milk using Western blot analysis and assay of proteolytic activity in acidic buffers. No other forms of cathepsin D were found. Two different polyclonal anti-procathepsin D antibodies were used for immunochemical detection of procathepsin D. Both antibodies we found to recognize rat procathepsin D. Proteolytic activity in acidic buffers was detected using a fluorogenic substrate specific for cathepsin D and was abolished by pepstatin A, a specific inhibitor of aspartic proteinases. This study represents third demonstration of presence of procathepsin D in mammal breast milk. Potential sources and physiological functions are discussed.
Abstract (in Czech)
Přítomnost prokatepsinu D (a nikoli jiných forem tohoto proteinu) byla detekována v krysím mléku pomocí několika různých technik. V práci jsou diskutovány možné fyziologické důsledky.
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