D 2002

Elimination voltammetry of short oligonucleotides

TRNKOVÁ, Libuše, František JELEN and Irena POSTBIEGLOVÁ

Basic information

Original name

Elimination voltammetry of short oligonucleotides

Authors

TRNKOVÁ, Libuše (203 Czech Republic, guarantor), František JELEN (203 Czech Republic) and Irena POSTBIEGLOVÁ (203 Czech Republic)

Edition

1. vyd. Frankfurt am Mai, Germany, Electrochemistry in Molecular and Microscopic Dimensions, 53rd Annual Meeting of the International Society of Electrochemistry, Book of Abstracts, p. 105-105, 2002

Publisher

Dechema e.V., Society for Chemical Engineering and Biotechnology,Frankfurt am Mai,Germany

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/02:00006480

Organization unit

Faculty of Informatics

Keywords in English

Oligonucleotides;Reduction of adenine and cytosine;Elimination voltammetry with linear scan;adsorption;interaction

Tags

Změněno: 31/5/2003 14:34, RNDr. JUDr. Vladimír Šmíd, CSc.

Abstract

V originále

Oligonucleotides containing adenines (A) or cytosines (C) yield reduction voltammetric peaks on mercury electrodes. The peak potentials of both bases are located very close to each other on the potential scale, so that usually only one voltammetric peak is observed. One signal only can be observed even when adsorptive transfer stripping voltammetry (AdTSV) is used. In the present work we have used elimination voltammetry with linear scan (EVLS) in connection with hanging mercury electrode, and shown that this electrochemical method is able to distinguish the two signals. EVLS is based on elimination of one or two selected currents from total currents measured by linear scan voltammetry. The function eliminating kinetic and charging currents, and conserving the diffusion current was calculated for the case of adsorbed electroactive substance. The theoretical elimination curve has a peak-counterpeak form. This elimination function was applied to the analysis of short oligonucleotides containing A and C in their molecules. The height and shift of EVLS signals were affected by pH, buffer components, the content of A and C, and the time of accumulation. Our results showed that the voltammetric signal of C can be separated from the signal of A, and that EVLS in connection with AdTSV is a promising tool for qualitative and quantitative studies of homo- and hetero-oligonucleotides.

Links

GA203/02/0422, research and development project
Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí
Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
IAA1163201, research and development project
Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles