2002
Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic
LOCHMAN, Jan, Omar ŠERÝ, Libor JANKOVSKÝ and Vladimír MIKEŠBasic information
Original name
Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic
Authors
LOCHMAN, Jan (203 Czech Republic, guarantor), Omar ŠERÝ (203 Czech Republic), Libor JANKOVSKÝ (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic)
Edition
Prague, Book of Abstracts of the congress EFPP, p. 7-7, 2002
Publisher
European Foundation for Plant Pathology
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/00216224:14310/02:00006494
Organization unit
Faculty of Science
Keywords in English
Armillaria; discrimination; ITS; PCR
Tags
Tags
International impact
Changed: 1/3/2007 17:24, prof. RNDr. Omar Šerý, Ph.D.
Abstract
V originále
More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens) using the PCR of the internal transcribed spacer (ITS). The amplificates were further analysed by RFLP (Alu I, Hinf I and Mbo I) and electrophoreticaly separated both on 3% agarose gel and by IEC- HPLC on TSKgel DEAE-NPR column (Supelco). Digestion with Hinf I allowed discrimination of all six species.
Links
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