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@inproceedings{405682, author = {Lochman, Jan and Šerý, Omar and Jankovský, Libor and Mikeš, Vladimír}, address = {Prague}, booktitle = {Book of Abstracts of the congress EFPP}, keywords = {Armillaria; discrimination; ITS; PCR}, language = {eng}, location = {Prague}, pages = {7-7}, publisher = {European Foundation for Plant Pathology}, title = {Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic}, year = {2002} }
TY - JOUR ID - 405682 AU - Lochman, Jan - Šerý, Omar - Jankovský, Libor - Mikeš, Vladimír PY - 2002 TI - Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic PB - European Foundation for Plant Pathology CY - Prague KW - Armillaria KW - discrimination KW - ITS KW - PCR N2 - More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens) using the PCR of the internal transcribed spacer (ITS). The amplificates were further analysed by RFLP (Alu I, Hinf I and Mbo I) and electrophoreticaly separated both on 3% agarose gel and by IEC- HPLC on TSKgel DEAE-NPR column (Supelco). Digestion with Hinf I allowed discrimination of all six species. ER -
LOCHMAN, Jan, Omar ŠERÝ, Libor JANKOVSKÝ and Vladimír MIKEŠ. Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic. In \textit{Book of Abstracts of the congress EFPP}. Prague: European Foundation for Plant Pathology, 2002, p.~7.
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