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@inproceedings{405684,
author = {Kašparovský, Tomáš and Milat, ML and Blein, JP and Mikeš, Vladimír},
address = {Prague},
booktitle = {Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci},
keywords = {ergposterol; plant protection; tobacco; elicitation},
language = {eng},
location = {Prague},
pages = {516-518},
publisher = {Institute of agricultural an food inforamtion},
title = {Ergosterol induces mobilization of internal calcium in tobacco cells},
year = {2002}
}
TY - JOUR
ID - 405684
AU - Kašparovský, Tomáš - Milat, ML - Blein, JP - Mikeš, Vladimír
PY - 2002
TI - Ergosterol induces mobilization of internal calcium in tobacco cells
PB - Institute of agricultural an food inforamtion
CY - Prague
KW - ergposterol
KW - plant protection
KW - tobacco
KW - elicitation
N2 - Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases.
ER -
KAŠPAROVSKÝ, Tomáš, ML MILAT, JP BLEIN and Vladimír MIKEŠ. Ergosterol induces mobilization of internal calcium in tobacco cells. In \textit{Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci}. Prague: Institute of agricultural an food inforamtion, 2002, p.~516-518. ISSN~1212-2580.
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