Analysis of Lignans in Schisandra Extract, Comparison between
CECand MEKC

D 2002

Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE et. al.

Basic information

Original name

Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC

Authors

KVASNIČKOVÁ, Leona (203 Czech Republic), Hana ŠTĚRBOVÁ (203 Czech Republic), Petra ŠEVČÍKOVÁ (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic, guarantor), Vladislav KAHLE (203 Czech Republic) and Jiří SLANINA (203 Czech Republic)

Edition

2002. vyd. Helsinki, proceeding of the 13th International Symposium on Capillary Electroseparation Techniques ITP 2002, p. 110-110, 2002

Publisher

University of Helsinki

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00006539

Organization unit

Faculty of Science

Keywords in English

Lignans; Schisandra; CEC; MEKC

Abstract

V originále

Schisandra chinensis BAILL. (Schisandraceae/Magnoliaceae) is a famous medicinal plant native to East Asia. It grows wild mainly in northeastern China, Korea and Japan and also in the most Eastern parts of Russia. The biologically active compounds are the lignans with an uncommon structure derived from dibenzo[a,c]cyclooctadiene. The lignans prevent liver injuries stimulate liver regeneration and also inhibit hepatocarcinogenesis and lipid peroxidation. Moreover several reports indicate, that the lignans have effects on human intellectual activity and physical performance. Recently, some dibenzo[a,c]cyclooctadiene lignans were identified as potent anti-HIV agent. Several analytical methods have been reported for the determinations of the lignans in Schisandra plant. Previously described methods include thin layer and high-performance liquid chromatographic techniques. Gas and high-performance liquid chromatography in combination with mass spectroscopy have also been demonstrated to be effective analytical method for the determination of the lignans in various samples. In this study we report capillary electrochromatography (CEC) in macroporous polyacrylamide columns and micellar electrokinetic capillary chromatography (MEKC) for the determination of several lignans - schizandrin, gomisin A, gomisin N and wuweizisu C, the major compounds of the Schisandra extracts. The reproducibilities of peak areas and migration times and linearities and limits of detection were investigated and compared. The results of quantitative analyses were compared as well, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by MEKC and with previous literature data.

Links

GA203/02/1447, research and development project

Name: Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Investor: Czech Science Foundation, Capillary electrochromatography in linear and planar microstructures using polymer beds

Citovat

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE and Jiří SLANINA. Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC. In proceeding of the 13th International Symposium on Capillary Electroseparation Techniques ITP 2002. 2002nd ed. Helsinki: University of Helsinki, 2002, p. 110.

@inproceedings{405934,
   author = {Kvasničková, Leona and Štěrbová, Hana and Ševčíková, Petra and Glatz, Zdeněk and Kahle, Vladislav and Slanina, Jiří},
   address = {Helsinki},
   booktitle = {proceeding of the 13th International Symposium on Capillary Electroseparation Techniques ITP 2002},
   edition = {2002},
   keywords = {Lignans; Schisandra; CEC; MEKC},
   language = {eng},
   location = {Helsinki},
   pages = {110-110},
   publisher = {University of Helsinki},
   title = {Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC},
   year = {2002}
}

TY  - JOUR
ID  - 405934
AU  - Kvasničková, Leona - Štěrbová, Hana - Ševčíková, Petra - Glatz, Zdeněk - Kahle, Vladislav - Slanina, Jiří
PY  - 2002
TI  - Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC
PB  - University of Helsinki
CY  - Helsinki
KW  - Lignans
KW  - Schisandra
KW  - CEC
KW  - MEKC
N2  - Schisandra chinensis BAILL. (Schisandraceae/Magnoliaceae) is a famous medicinal plant native to East Asia. It grows wild mainly in northeastern China, Korea and Japan and also in the most Eastern parts of Russia. The biologically active compounds are the lignans with an uncommon structure derived from dibenzo[a,c]cyclooctadiene. The lignans prevent liver injuries stimulate liver regeneration and also inhibit hepatocarcinogenesis and lipid peroxidation. Moreover several reports indicate, that the lignans have effects on human intellectual activity and physical performance. Recently, some dibenzo[a,c]cyclooctadiene lignans were identified as potent anti-HIV agent. Several analytical methods have been reported for the determinations of the lignans in Schisandra plant. Previously described methods include thin layer and high-performance liquid chromatographic techniques. Gas and high-performance liquid chromatography in combination with mass spectroscopy have also been demonstrated to be effective analytical method for the determination of the lignans in various samples. In this study we report capillary electrochromatography (CEC) in macroporous polyacrylamide columns and micellar electrokinetic capillary chromatography (MEKC) for the determination of several lignans - schizandrin, gomisin A, gomisin N and wuweizisu C, the major compounds of the Schisandra extracts. The reproducibilities of peak areas and migration times and linearities and limits of detection were investigated and compared. The results of quantitative analyses were compared as well, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by MEKC and with previous literature data.
ER  -

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE and Jiří SLANINA. Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC. In \textit{proceeding of the 13th International Symposium on Capillary Electroseparation Techniques ITP 2002}. 2002nd ed. Helsinki: University of Helsinki, 2002, p.~110.

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