J 2002

3D Structure of the human genome: Order in randomness

KOZUBEK, Stanislav, Emilie LUKÁŠOVÁ, Pavla JIRSOVÁ, Irena KOUTNÁ, Michal KOZUBEK et. al.

Basic information

Original name

3D Structure of the human genome: Order in randomness

Authors

KOZUBEK, Stanislav (203 Czech Republic, belonging to the institution), Emilie LUKÁŠOVÁ (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic, belonging to the institution), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Alena GAŇOVÁ (203 Czech Republic, belonging to the institution), Eva BÁRTOVÁ (203 Czech Republic, belonging to the institution), Martin FALK (203 Czech Republic) and Renata TASLEROVÁ (203 Czech Republic, belonging to the institution)

Edition

Chromosoma, Berlin, Springer-Verlag, 2002, 0009-5915

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.829

RIV identification code

RIV/00216224:14330/02:00006554

Organization unit

Faculty of Informatics

UT WoS

000180569800005

Keywords in English

human genome structure; interphase cell nuclei

Tags

Tags

International impact, Reviewed
Změněno: 24/8/2012 12:23, doc. RNDr. Martin Falk, Ph.D.

Abstract

V originále

A complex study of the spatial arrangement of different genetic elements (genes, centromeres and chromosomal domains) in the cell nucleus is presented and the principles of this arrangement are discussed. We show that the radial location of genetic elements in the three-dimensional (3D) space between the center of the nucleus and the nuclear membrane is element specific and dependent on the position of the element on the chromosome. In contrast, mutual angular positioning of both homologous and heterologous genetic elements is, in the majority of cases, random. In several cases, tethering of heterologous genetic elements was observed. This close proximity of specific loci may be responsible for their mutual rearrangement and the development of cancer. Comparison of our results with transcriptome maps shows that the nuclear location of chromosomal domains with highly expressed genes is more central when compared with chromosomes with low expression. The higher-order chromatin structure is strikingly similar in various human cell types, which correlates with the fact that the profiles of gene expression are also similar.

Links

GA301/01/0186, research and development project
Name: Studium lokální kontroly exprese genů pomocí spektrální mikroskopie a analýzy obrazu
Investor: Czech Science Foundation, Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques
IBS5004010, research and development project
Name: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NC5955, research and development project
Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů.
Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.