Detailed Information on Publication Record
2002
Nuclear structure and gene activity in human differentiated cells
BÁRTOVÁ, Eva, Stanislav KOZUBEK, Pavla JIRSOVÁ, Michal KOZUBEK, Hana GAJOVÁ et. al.Basic information
Original name
Nuclear structure and gene activity in human differentiated cells
Authors
BÁRTOVÁ, Eva (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Michal KOZUBEK (203 Czech Republic, guarantor), Hana GAJOVÁ (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Alena GAŇOVÁ (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic) and Michael HAUSMANN (276 Germany)
Edition
Journal of Structural Biology, San Diego,USA, Academic Press, 2002, 1047-8477
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.194
RIV identification code
RIV/00216224:14330/02:00006555
Organization unit
Faculty of Informatics
UT WoS
000179266300002
Keywords in English
human genome structure; interphase cell nuclei
Tags
International impact, Reviewed
Změněno: 7/5/2010 15:58, prof. RNDr. Michal Kozubek, Ph.D.
Abstract
V originále
The nuclear arrangement of the ABL, c-MYC, and RB1 genes was quantitatively investigated in human undifferentiated HL-60 cells and in a terminally differentiated population of human granulocytes. The ABL gene was expressed in both cell types, the c-MYC gene was active in HL-60 cells and down-regulated in granulocytes, and expression of the RB1 gene was undetectable in HL-60 cells but up-regulated in granulocytes. The distances of these genes to the nuclear center (membrane), to the center of the corresponding chromosome territory, and to the nearest centromere were determined. During granulopoesis, the majority of selected genetic structures were repositioned closer to the nuclear periphery. The nuclear reposition of the genes studied did not correlate with the changes of their expression. In both cell types, the c-MYC and RB1 genes were located at the periphery of the chromosome territories regardless of their activity. The centromeres of chromosomes 8 and 13 were always positioned more centrally within the chromosome territory than the studied genes. Close spatial proximity of the c-MYC and RB1 genes with centromeric heterochromatin, forming the chromocenters, correlated with gene activity, although the nearest chromocenter of the silenced RB1 gene did not involve centromeric heterochromatin of chromosome 13 where the given gene is localized. In addition, the role of heterochromatin in gene silencing was studied in retinoblastoma cells. In these differentiated tumor cells, one copy of the RB1 gene was positioned near the heterochromatic chromosome X, and reduced RB1 gene activity was observed. In the experiments presented here, we provide evidence that the regulation of gene activity during important cellular processes such as differentiation or carcinogenesis may be realized through heterochromatin-mediated gene silencing.
Links
| GA301/01/0186, research and development project |
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| IAB5004102, research and development project |
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| IBS5004010, research and development project |
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| MSM 143300002, plan (intention) |
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