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@article{406043,
author = {Bártová, Eva and Kozubek, Stanislav and Jirsová, Pavla and Kozubek, Michal and Gajová, Hana and Lukášová, Emilie and Skalníková, Magdalena and Gaňová, Alena and Koutná, Irena and Hausmann, Michael},
article_location = {San Diego,USA},
article_number = {2},
keywords = {human genome structure; interphase cell nuclei},
language = {eng},
issn = {1047-8477},
journal = {Journal of Structural Biology},
title = {Nuclear structure and gene activity in human differentiated cells},
volume = {139},
year = {2002}
}
TY - JOUR
ID - 406043
AU - Bártová, Eva - Kozubek, Stanislav - Jirsová, Pavla - Kozubek, Michal - Gajová, Hana - Lukášová, Emilie - Skalníková, Magdalena - Gaňová, Alena - Koutná, Irena - Hausmann, Michael
PY - 2002
TI - Nuclear structure and gene activity in human differentiated cells
JF - Journal of Structural Biology
VL - 139
IS - 2
SP - 76
EP - 76
PB - Academic Press
SN - 10478477
KW - human genome structure
KW - interphase cell nuclei
N2 - The nuclear arrangement of the ABL, c-MYC, and RB1 genes was quantitatively investigated in human undifferentiated HL-60 cells and in a terminally differentiated population of human granulocytes. The ABL gene was expressed in both cell types, the c-MYC gene was active in HL-60 cells and down-regulated in granulocytes, and expression of the RB1 gene was undetectable in HL-60 cells but up-regulated in granulocytes. The distances of these genes to the nuclear center (membrane), to the center of the corresponding chromosome territory, and to the nearest centromere were determined. During granulopoesis, the majority of selected genetic structures were repositioned closer to the nuclear periphery. The nuclear reposition of the genes studied did not correlate with the changes of their expression. In both cell types, the c-MYC and RB1 genes were located at the periphery of the chromosome territories regardless of their activity. The centromeres of chromosomes 8 and 13 were always positioned more centrally within the chromosome territory than the studied genes. Close spatial proximity of the c-MYC and RB1 genes with centromeric heterochromatin, forming the chromocenters, correlated with gene activity, although the nearest chromocenter of the silenced RB1 gene did not involve centromeric heterochromatin of chromosome 13 where the given gene is localized. In addition, the role of heterochromatin in gene silencing was studied in retinoblastoma cells. In these differentiated tumor cells, one copy of the RB1 gene was positioned near the heterochromatic chromosome X, and reduced RB1 gene activity was observed. In the experiments presented here, we provide evidence that the regulation of gene activity during important cellular processes such as differentiation or carcinogenesis may be realized through heterochromatin-mediated gene silencing.
ER -
BÁRTOVÁ, Eva, Stanislav KOZUBEK, Pavla JIRSOVÁ, Michal KOZUBEK, Hana GAJOVÁ, Emilie LUKÁŠOVÁ, Magdalena SKALNÍKOVÁ, Alena GAŇOVÁ, Irena KOUTNÁ and Michael HAUSMANN. Nuclear structure and gene activity in human differentiated cells. \textit{Journal of Structural Biology}. San Diego,USA: Academic Press, 2002, vol.~139, No~2, p.~76-89. ISSN~1047-8477.
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