Detailed Information on Publication Record
2002
The 3D structure of human chromosomes in cell nuclei
LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Martin FALK, Jana AMRICHOVÁ et. al.Basic information
Original name
The 3D structure of human chromosomes in cell nuclei
Authors
LUKÁŠOVÁ, Emilie (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic, belonging to the institution), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Martin FALK (203 Czech Republic) and Jana AMRICHOVÁ (203 Czech Republic, belonging to the institution)
Edition
Chromosome Research, Dordrecht, Kluwer Academic, 2002, 0967-3849
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.828
RIV identification code
RIV/00216224:14330/02:00006557
Organization unit
Faculty of Informatics
UT WoS
000178954300002
Keywords in English
chromosome structure; confocal microscopy; mathematical models; nuclear architecture
Tags
Tags
International impact, Reviewed
Změněno: 24/8/2012 12:24, doc. RNDr. Martin Falk, Ph.D.
Abstract
V originále
The spatial arrangement of some genetic elements relative to chromosome territories and in parallel with the cell nucleus was investigated in human lymphocytes. The structure of the chromosome territories was studied in chromosomes containing regions (clusters) of highly expressed genes (HSA 9, 17) and those without such clusters (HSA 8, 13). In chromosomes containing highly expressed regions, the elements pertaining to these regions were found close to the centre of the nucleus on the inner sides of chromosome territories; those pertaining to regions with low expression were localized close to the nuclear membrane on the opposite sides of the territories. In chromosomes with generally low expression (HSA 8, 13), the elements investigated were found symmetrically distributed over the territories. Based on the investigations of the chromosome structure, the following conclusions are suggested: (1) Chromosome territories have a non-random internal 3D structure with defined average mutual positions between elements. For example, RARalpha, TP53 and Iso-q of HSA 17 are nearer to each other than they are to the HSA 17 centromere. (2) The structure of a chromosome territory reflects the number and chromosome location of clusters of highly expressed genes. (3) Chromosome territories behave to some extent as solid bodies: if the territory is found closer to the nuclear centre, the individual genetic elements of this chromosome are also found, on average, closer the centre of the nucleus. (4) The positions of centromeres are, on average, nearer to the fluorescence weight centre of the territory (FWCT) than to genes. (5) Active genes are not found near the centromeres of their own territory. A simple model of the structure of chromosome territory is proposed.
Links
| GA301/01/0186, research and development project |
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| IAA1065203, research and development project |
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| IBS5004010, research and development project |
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| MSM 143300002, plan (intention) |
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| NC5955, research and development project |
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