Multiplex PCR for detection of Staphylococcus aureus prophages

D 2002

Multiplex PCR for detection of Staphylococcus aureus prophages

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ et. al.

Basic information

Original name

Multiplex PCR for detection of Staphylococcus aureus prophages

Authors

PANTŮČEK, Roman (203 Czech Republic, guarantor), Eva ORÁČOVÁ (203 Czech Republic), Veronika KVARDOVÁ (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Petr PETRÁŠ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Tsukuba, Japan, 10th International Symposium on Staphylococci and Staphylococcal Infections, p. 66-66, 2002

Publisher

Juntendo University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Japan

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00006633

Organization unit

Faculty of Science

Keywords in English

Bacterial Typing Techniques; DNA; Bacteriophages; Staphylococcus aureus; Lysogeny; Multiplex PCR; Prophage detection

Abstract

V originále

Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2000,Can.J.Microbiol.46:1066-1076). Conclusions.In view of significant differences in the prophage content between S.aureus closely related isolates,prophage profiling is recommended as a highly sensitive method for strain discrimination.

Links

GA301/02/1505, research and development project

Name: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Czech Science Foundation, Molecular diagnostic, epidemiology and classification of pathogenic Gram positive cocci

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ and Jiří DOŠKAŘ. Multiplex PCR for detection of Staphylococcus aureus prophages. In 10th International Symposium on Staphylococci and Staphylococcal Infections. Tsukuba, Japan: Juntendo University, 2002, p. 66.

@inproceedings{406333,
   author = {Pantůček, Roman and Oráčová, Eva and Kvardová, Veronika and Růžičková, Vladislava and Petráš, Petr and Doškař, Jiří},
   address = {Tsukuba, Japan},
   booktitle = {10th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Bacterial Typing Techniques; DNA; Bacteriophages; Staphylococcus aureus; Lysogeny; Multiplex PCR; Prophage detection},
   language = {eng},
   location = {Tsukuba, Japan},
   pages = {66-66},
   publisher = {Juntendo University},
   title = {Multiplex PCR for detection of Staphylococcus aureus prophages},
   year = {2002}
}

TY  - JOUR
ID  - 406333
AU  - Pantůček, Roman - Oráčová, Eva - Kvardová, Veronika - Růžičková, Vladislava - Petráš, Petr - Doškař, Jiří
PY  - 2002
TI  - Multiplex PCR for detection of Staphylococcus aureus prophages
PB  - Juntendo University
CY  - Tsukuba, Japan
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Bacteriophages
KW  - Staphylococcus aureus
KW  - Lysogeny
KW  - Multiplex PCR
KW  - Prophage detection
N2  - Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2000,Can.J.Microbiol.46:1066-1076). Conclusions.In view of significant differences in the prophage content between S.aureus closely related isolates,prophage profiling is recommended as a highly sensitive method for strain discrimination.
ER  -

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ and Jiří DOŠKAŘ. Multiplex PCR for detection of $<$I$>$Staphylococcus aureus$<$/I$>$ prophages. In \textit{10th International Symposium on Staphylococci and Staphylococcal Infections}. Tsukuba, Japan: Juntendo University, 2002, p.~66.

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