Spectrin-like protein in yeasts

D 2002

Spectrin-like protein in yeasts

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ and Augustin SVOBODA

Basic information

Original name

Spectrin-like protein in yeasts

Authors

SLANINOVÁ, Iva (203 Czech Republic, guarantor), Alena HOLUBÁŘOVÁ (203 Czech Republic) and Augustin SVOBODA (203 Czech Republic)

Edition

Bratislava, Sborník XXX th Annual Conference on yeasts, p. 36-36, 2002

Publisher

Československá mikrobiologická společnost

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/02:00006734

Organization unit

Faculty of Medicine

Keywords in English

actin; spectrin; yeast; cytoskeleton

Abstract

V originále

Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface.

Links

GA204/00/0394, research and development project

Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

GA204/99/D025, research and development project

Name: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization

Citovat

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ and Augustin SVOBODA. Spectrin-like protein in yeasts. In Sborník XXX th Annual Conference on yeasts. Bratislava: Československá mikrobiologická společnost, 2002, p. 36.

@inproceedings{406736,
   author = {Slaninová, Iva and Holubářová, Alena and Svoboda, Augustin},
   address = {Bratislava},
   booktitle = {Sborník XXX th Annual Conference on yeasts},
   keywords = {actin; spectrin; yeast; cytoskeleton},
   language = {eng},
   location = {Bratislava},
   pages = {36-36},
   publisher = {Československá mikrobiologická společnost},
   title = {Spectrin-like protein in yeasts},
   year = {2002}
}

TY  - JOUR
ID  - 406736
AU  - Slaninová, Iva - Holubářová, Alena - Svoboda, Augustin
PY  - 2002
TI  - Spectrin-like protein in yeasts
PB  - Československá mikrobiologická společnost
CY  - Bratislava
KW  - actin
KW  - spectrin
KW  - yeast
KW  - cytoskeleton
N2  - Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface.
ER  -

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ and Augustin SVOBODA. Spectrin-like protein in yeasts. In \textit{Sborník XXX th Annual Conference on yeasts}. Bratislava: Československá mikrobiologická společnost, 2002, p.~36.

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