Computer-assisted quantitative immunohistochemistry of the
extracellular matrix in the dorsal and ventral spinal roots
with the use of a Lucia software

J 2002

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ

Basic information

Original name

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor), Ivana SVÍŽENSKÁ (203 Czech Republic) and Ilona KLUSÁKOVÁ (203 Czech Republic)

Edition

Acta histochemica, Jena, Urban&Fischer Verlag, 2002, 0065-1281

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.867

RIV identification code

RIV/00216224:14110/02:00006843

Organization unit

Faculty of Medicine

UT WoS

000180301000011

Keywords in English

endoneurium; extracellular; matrix; immunofluorescence; image analysis

Abstract

ORIG CZ

V originále

The endoneurial ECM is produced by Schwann cells and fibroblasts under the control of the axons. The dorsal and ventral spinal roots contain different types of axons, but no information is available about differences of their ECM content. The present paper demonstrates a comparison of immunofluorescence intensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were incubated simultaneously for indirect immunofluorescence detection of studied epitopes. The brightness of immunofluorescence staining was assessed by computer-assisted image analysis (Lucia G, Laboratory Imaging Ltd., Prague) using interactive segmentation of digitized pictures to select the areas of measurement. The results revealed quantitative differences of the endoneurial ECM content of the spinal dorsal and ventral roots probably corresponding to the presence of different types of axons. The ECM composition of the endoneurium in dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.

In Czech

Endoneuriální ECM je produkována Schwannovými buňkami a fibroblasty pod kontrolou axonů. Složení endoneuriální ECM bylo studováno kvantitativní imunohistochemii.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

Citovat

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software. Acta histochemica. Jena: Urban&Fischer Verlag, 2002, vol. 104, No 4, p. 371-374. ISSN 0065-1281.

@article{407044,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona},
   article_location = {Jena},
   article_number = {4},
   keywords = {endoneurium; extracellular; matrix; immunofluorescence; image analysis},
   language = {eng},
   issn = {0065-1281},
   journal = {Acta histochemica},
   title = {Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software},
   volume = {104},
   year = {2002}
}

TY  - JOUR
ID  - 407044
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona
PY  - 2002
TI  - Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software
JF  - Acta histochemica
VL  - 104
IS  - 4
SP  - 371
EP  - 371
PB  - Urban&Fischer Verlag
SN  - 00651281
KW  - endoneurium
KW  - extracellular
KW  - matrix
KW  - immunofluorescence
KW  - image analysis
N2  - The endoneurial ECM is produced by Schwann cells and fibroblasts under the control of the axons. The dorsal and ventral spinal roots contain different types of axons, but no information is available about differences of their ECM content. The present paper demonstrates a comparison of immunofluorescence intensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were incubated simultaneously for indirect immunofluorescence detection of studied epitopes. The brightness of immunofluorescence staining was assessed by computer-assisted image analysis (Lucia G, Laboratory Imaging Ltd., Prague) using interactive segmentation of digitized pictures to select the areas of measurement. The results revealed quantitative differences of the endoneurial ECM content of the spinal dorsal and ventral roots probably corresponding to the presence of different types of axons. The ECM composition of the endoneurium in dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
ER  -

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software. \textit{Acta histochemica}. Jena: Urban\&{}Fischer Verlag, 2002, vol.~104, No~4, p.~371-374. ISSN~0065-1281.

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