Identification of Salmonella enterica serovar Typhimurium genes
associated with growth suppression in stationary-phase nutrient
broth cultures and in the chicken intestine

J 2002

Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine

RYCHLÍK, Ivan, Martin GERALD, Ulrich METHNER, Margaret LOVELL, Lenka CRDOVÁ et. al.

Basic information

Original name

Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine

Authors

RYCHLÍK, Ivan (203 Czech Republic, guarantor), Martin GERALD (276 Germany), Ulrich METHNER (276 Germany), Margaret LOVELL (826 United Kingdom of Great Britain and Northern Ireland), Lenka CRDOVÁ (203 Czech Republic), Alena SBKOVÁ (203 Czech Republic), Mojmír SEVCIK (203 Czech Republic), Jiri DAMBORSKÝ (203 Czech Republic) and Paul BARROW (826 United Kingdom of Great Britain and Northern Ireland)

Edition

Archives of Microbiology, 2002, 0302-8933

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.903

RIV identification code

RIV/00216224:14310/02:00006912

Organization unit

Faculty of Science

UT WoS

000179674200005

Keywords in English

VIRULENCE; RESISTANCE; SALMONELLA; YHJH GENE; MUTANT

Abstract

V originále

Over 2,800 Tn5 mutants of Salmonella enterica serovar Typhimurium have been screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant but otherwise isogenic S. Typhimurium strain, when this was added to 24 hour LB broth cultures of the mutants. Selected growth non-suppressive (GNS) mutants were defective in respiration (insertions in arcA and fnr), amino acid biosynthesis (aroA and aroD), nutrient uptake and its regulation (tdcC and crp), and in chemotaxis (fliD). In the last GNS mutant, the transposon inactivated yhjH, an ORF with unknown function which shows homology to di-guanylate cyclase and novel two-component signal transduction proteins. In newly-hatched chickens, all the mutants, with the exception of the fliD mutant, were also unable to suppress colonisation of the alimentary tract by the parent strain inoculated one day later. Defined mutations in luxS or sdiA, genes which contribute to quorum sensing in S. Typhimurium, had no effect on the stationary-phase growth suppression. A transcriptional fusion was constructed which indicated that the yhjH was moderately expressed in the exponential phase of growth and up-regulated upon entry into stationary phase. Expression of yhjH was also considerably suppressed by the addition of supernatant from a 24 hour stationary-phase S. Typhimurium culture suggesting that it belongs to a new sensing and signalling regulatory pathway in S. Typhimurium.

Links

LN00A016, research and development project

Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center

Citovat

RYCHLÍK, Ivan, Martin GERALD, Ulrich METHNER, Margaret LOVELL, Lenka CRDOVÁ, Alena SBKOVÁ, Mojmír SEVCIK, Jiri DAMBORSKÝ and Paul BARROW. Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine. Archives of Microbiology. 2002, vol. 178, No 6, p. 411-420. ISSN 0302-8933.

@article{407281,
   author = {Rychlík, Ivan and Gerald, Martin and Methner, Ulrich and Lovell, Margaret and Crdová, Lenka and Sbková, Alena and Sevcik, Mojmír and Damborský, Jiri and Barrow, Paul},
   article_number = {6},
   keywords = {VIRULENCE; RESISTANCE; SALMONELLA; YHJH GENE; MUTANT},
   language = {eng},
   issn = {0302-8933},
   journal = {Archives of Microbiology},
   title = {Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine},
   url = {http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/archmicro02b.html},
   volume = {178},
   year = {2002}
}

TY  - JOUR
ID  - 407281
AU  - Rychlík, Ivan - Gerald, Martin - Methner, Ulrich - Lovell, Margaret - Crdová, Lenka - Sbková, Alena - Sevcik, Mojmír - Damborský, Jiri - Barrow, Paul
PY  - 2002
TI  - Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine
JF  - Archives of Microbiology
VL  - 178
IS  - 6
SP  - 411
EP  - 411
SN  - 03028933
KW  - VIRULENCE
KW  - RESISTANCE
KW  - SALMONELLA
KW  - YHJH GENE
KW  - MUTANT
UR  - http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/archmicro02b.html
N2  - Over 2,800 Tn5 mutants of Salmonella enterica serovar Typhimurium have been screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant but otherwise isogenic S. Typhimurium strain, when this was added to 24 hour LB broth cultures of the mutants. Selected growth non-suppressive (GNS) mutants were defective in respiration (insertions in arcA and fnr), amino acid biosynthesis (aroA and aroD), nutrient uptake and its regulation (tdcC and crp), and in chemotaxis (fliD). In the last GNS mutant, the transposon inactivated yhjH, an ORF with unknown function which shows homology to di-guanylate cyclase and novel two-component signal transduction proteins. In newly-hatched chickens, all the mutants, with the exception of the fliD mutant, were also unable to suppress colonisation of the alimentary tract by the parent strain inoculated one day later. Defined mutations in luxS or sdiA, genes which contribute to quorum sensing in S. Typhimurium, had no effect on the stationary-phase growth suppression. A transcriptional fusion was constructed which indicated that the yhjH was moderately expressed in the exponential phase of growth and up-regulated upon entry into stationary phase. Expression of yhjH was also considerably suppressed by the addition of supernatant from a 24 hour stationary-phase S. Typhimurium culture suggesting that it belongs to a new sensing and signalling regulatory pathway in S. Typhimurium.
ER  -

RYCHLÍK, Ivan, Martin GERALD, Ulrich METHNER, Margaret LOVELL, Lenka CRDOVÁ, Alena SBKOVÁ, Mojmír SEVCIK, Jiri DAMBORSKÝ and Paul BARROW. Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine. \textit{Archives of Microbiology}. 2002, vol.~178, No~6, p.~411-420. ISSN~0302-8933.

Detailed Information on Publication Record