Recovery of tobacco cells from cadmium stress is accompanied by
DNA repair and increased telomerase activity

J 2002

Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity

FOJTOVÁ, Miloslava, Jana FULNEČKOVÁ, Jiří FAJKUS and Aleš KOVAŘÍK

Basic information

Original name

Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity

Authors

FOJTOVÁ, Miloslava (203 Czech Republic), Jana FULNEČKOVÁ (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, guarantor) and Aleš KOVAŘÍK (203 Czech Republic)

Edition

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2002, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.852

RIV identification code

RIV/00216224:14310/02:00006960

Organization unit

Faculty of Science

UT WoS

000178957500003

Keywords in English

apoptosis; cadmium; DNA domain fragmentation; telomerase; tobacco BY-2 cells

Abstract

V originále

It has been shown previously that apoptosis of tobacco cells induced by cadmium ions shows a relatively long lag period between exposure and ceel death. This lag phase lasts for 3 d in TBY-2 cell cultures and is characterized by the maintenance of full cell viability despite extensive fragmentation of DNA into pieces of chromatin loop size. Experiments reported here demonstrate that cell death can be prevented if CdSO4 is removed from the growth medium during the lag phase, suggesting that an irreversible apoptotic trigger is delivered within 24 h, between the third and fourth days of cadmium treatment. The post-cadmium recovery phase was characterized by DNA repair at the level of 50-200 kb and increased telomerase activity. Analysis of high-molecular-weight DNA by pulsed-field-gel electrophoresis revealed that the majority of DNA strand breaks was repaired within 48 h after cadmium withdrawal. Telomerase activity increased 2.5-fold in the recovery phase, but elevated levels were also found in cell extracts from apoptotic cells suggesting that telomerase might be associated with DNA repair, but it is not capable of inhibiting ongoing apoptosis. Limited exposure of TBY-2 cells to cadmium elicits non-random DNA damage of relatively high magnitude that can be repaired. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient genotoxic stress.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

FOJTOVÁ, Miloslava, Jana FULNEČKOVÁ, Jiří FAJKUS and Aleš KOVAŘÍK. Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2002, roč. 53, No 378, p. 2151-2158. ISSN 0022-0957.

@article{407403,
   author = {Fojtová, Miloslava and Fulnečková, Jana and Fajkus, Jiří and Kovařík, Aleš},
   article_location = {Oxford},
   article_number = {378},
   keywords = {apoptosis; cadmium; DNA domain fragmentation; telomerase; tobacco BY-2 cells},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity},
   volume = {roč. 53},
   year = {2002}
}

TY  - JOUR
ID  - 407403
AU  - Fojtová, Miloslava - Fulnečková, Jana - Fajkus, Jiří - Kovařík, Aleš
PY  - 2002
TI  - Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity
JF  - Journal of Experimental Botany
VL  - roč. 53
IS  - 378
SP  - 2151
EP  - 2151
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - apoptosis
KW  - cadmium
KW  - DNA domain fragmentation
KW  - telomerase
KW  - tobacco BY-2 cells
N2  - It has been shown previously that apoptosis of tobacco cells induced by cadmium ions shows a relatively long lag period between exposure and ceel death. This lag phase lasts for 3 d in TBY-2 cell cultures and is characterized by the maintenance of full cell viability despite extensive fragmentation of DNA into pieces of chromatin loop size. Experiments reported here demonstrate that cell death can be prevented if CdSO4 is removed from the growth medium during the lag phase, suggesting that an irreversible apoptotic trigger is delivered within 24 h, between the third and fourth days of cadmium treatment. The post-cadmium recovery phase was characterized by DNA repair at the level of 50-200 kb and increased telomerase activity. Analysis of high-molecular-weight DNA by pulsed-field-gel electrophoresis revealed that the majority of DNA strand breaks was repaired within 48 h after cadmium withdrawal. Telomerase activity increased 2.5-fold in the recovery phase, but elevated levels were also found in cell extracts from apoptotic cells suggesting that telomerase might be associated with DNA repair, but it is not capable of inhibiting ongoing apoptosis. Limited exposure of TBY-2 cells to cadmium elicits non-random DNA damage of relatively high magnitude that can be repaired. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient genotoxic stress.
ER  -

FOJTOVÁ, Miloslava, Jana FULNEČKOVÁ, Jiří FAJKUS and Aleš KOVAŘÍK. Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2002, roč. 53, No~378, p.~2151-2158. ISSN~0022-0957.

Detailed Information on Publication Record