Genome analysis of polyvalent staphylococcal bacteriophage 812

J 2002

Genome analysis of polyvalent staphylococcal bacteriophage 812

KAŠPÁREK, Petr, Jiří DOŠKAŘ and Roman PANTŮČEK

Basic information

Original name

Genome analysis of polyvalent staphylococcal bacteriophage 812

Authors

KAŠPÁREK, Petr (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic, guarantor) and Roman PANTŮČEK (203 Czech Republic)

Edition

Scripta medica (Brno), Brno, Masaryk University, Faculty of Medicine, 2002, 1211-3595

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00007080

Organization unit

Faculty of Science

Keywords in English

Bacteriophage; Staphylococcus; DNA; Bacteriophage Therapy; Endolysin Gene; Genome Mapping

Abstract

V originále

The phage 812 is a polyvalent virulent bacteriophage belonging to the family Myoviridae. It shows strong lytic activity on a broad host range of Staphylococcus aureus subsp.aureus strains as well as on some other staphylococcal species.The phage 812 is closely related to staphylococcal phages U16,SK311 and SK131 classified with the phage species Twort. The object of our study is the analysis of genome regions exhibiting chromosome rearrangements (insertions,deletions and inversions)in the host-range mutants of the phage 812.This analysis should contribute to the explanation of molecular mechanisms leading to the host range changes.Part of this study is focused on identification and characterization of genes and sequences of the phage 812 presumably relating to the host range changes. We determined the nucleotide sequence of a 913 bp insertion identified in the restriction fragment PstI-O of the host-range mutant 812b. The putative gene coding for the major capsid protein of the phage 812 has been found.This protein shows 65 %identity with the major capsid protein of the phage A511 growing on Listeria monocytogenes strains. Hybridization probe prepared from the intron sequences of phage Twort does not hybridize with the phage 812 DNA,nor with that of its host-range mutants,which indicates absence of the introns in these phages.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

KAŠPÁREK, Petr, Jiří DOŠKAŘ and Roman PANTŮČEK. Genome analysis of polyvalent staphylococcal bacteriophage 812. Scripta medica (Brno). Brno: Masaryk University, Faculty of Medicine, 2002, vol. 75, No 1, p. 39. ISSN 1211-3595.

@article{407798,
   author = {Kašpárek, Petr and Doškař, Jiří and Pantůček, Roman},
   article_location = {Brno},
   article_number = {1},
   keywords = {Bacteriophage; Staphylococcus; DNA; Bacteriophage Therapy; Endolysin Gene; Genome Mapping},
   language = {eng},
   issn = {1211-3595},
   journal = {Scripta medica (Brno)},
   title = {Genome analysis of polyvalent staphylococcal bacteriophage 812},
   volume = {75},
   year = {2002}
}

TY  - JOUR
ID  - 407798
AU  - Kašpárek, Petr - Doškař, Jiří - Pantůček, Roman
PY  - 2002
TI  - Genome analysis of polyvalent staphylococcal bacteriophage 812
JF  - Scripta medica (Brno)
VL  - 75
IS  - 1
SP  - 39
EP  - 39
PB  - Masaryk University, Faculty of Medicine
SN  - 12113595
KW  - Bacteriophage
KW  - Staphylococcus
KW  - DNA
KW  - Bacteriophage Therapy
KW  - Endolysin Gene
KW  - Genome Mapping
N2  - The phage 812 is a polyvalent virulent bacteriophage belonging to the family Myoviridae. It shows strong lytic activity on a broad host range of Staphylococcus aureus subsp.aureus strains as well as on some other staphylococcal species.The phage 812 is closely related to staphylococcal phages U16,SK311 and SK131 classified with the phage species Twort. The object of our study is the analysis of genome regions exhibiting chromosome rearrangements (insertions,deletions and inversions)in the host-range mutants of the phage 812.This analysis should contribute to the explanation of molecular mechanisms leading to the host range changes.Part of this study is focused on identification and characterization of genes and sequences of the phage 812 presumably relating to the host range changes. We determined the nucleotide sequence of a 913 bp insertion identified in the restriction fragment PstI-O of the host-range mutant 812b. The putative gene coding for the major capsid protein of the phage 812 has been found.This protein shows 65 %identity with the major capsid protein of the phage A511 growing on Listeria monocytogenes strains. Hybridization probe prepared from the intron sequences of phage Twort does not hybridize with the phage 812 DNA,nor with that of its host-range mutants,which indicates absence of the introns in these phages.
ER  -

KAŠPÁREK, Petr, Jiří DOŠKAŘ and Roman PANTŮČEK. Genome analysis of polyvalent staphylococcal bacteriophage 812. \textit{Scripta medica (Brno)}. Brno: Masaryk University, Faculty of Medicine, 2002, vol.~75, No~1, p.~39. ISSN~1211-3595.

Detailed Information on Publication Record