Biochemical and molecular taxonomy of gram-positive cocci
isolated from skin of llamas

a 2001

Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas

DOŠKAŘOVÁ, Sylva, Ivo SEDLÁČEK and Roman PANTŮČEK

Basic information

Original name

Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas

Authors

DOŠKAŘOVÁ, Sylva (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, guarantor) and Roman PANTŮČEK (203 Czech Republic)

Edition

2001

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00007081

Organization unit

Faculty of Science

Keywords in English

Bacterial Typing Techniques; DNA; Macrococcus; classification; Llama; Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA

Abstract

V originále

Macrococci are gram positive,catalase and oxidase positive cocci inhabiting skin of horses and ponnies. Their pathogenicity is unknown. The genus Macrococcus was earmarked from the genus Staphylococcus and described in 1998. Eight strains isolated from the skin of Lama glama breeded in the ZOO in Brno were described and characterized in this work. All strains studied were gram positive and catalase and oxidase positive, mostly orange-pigmented cocci. Their biochemical traits were in full agreement with the description of the genus Macrococcus. They differ from all hitherto described macrococci by their positive nitrate reduction,negative aesculin and DNA hydrolyses and negative glycerol acidification. The GC content of genomic DNAs were 45.1 - 52.2 %. Following molecular-genetic methods were done to clarify their taxonomic position: ribotyping,macrorestriction analysis and 16S rRNA gene sequencing. All phenotype and genotype analysis results obtained divided studied strains into two homegeneous groups and confirmed that they represent strains of the genus Macrococcus. Moreover,all strains were clearly dictiguished from all hitherto described macrococcal species. Our results imply that they represent two new species of the genus Macrococcus. This suggestion will be confirmed by DNA-DNA hybridisations. All strains studied were deposited in the Czech Collection of Microorganisms as Macrococcus sp. CCM 4808, CCM 4815.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

DOŠKAŘOVÁ, Sylva, Ivo SEDLÁČEK and Roman PANTŮČEK. Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas. 2001.

@proceedings{407801,
   author = {Doškařová, Sylva and Sedláček, Ivo and Pantůček, Roman},
   keywords = {Bacterial Typing Techniques; DNA; Macrococcus; classification; Llama; Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA},
   language = {eng},
   title = {Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas},
   year = {2001}
}

TY  - CONF
ID  - 407801
AU  - Doškařová, Sylva - Sedláček, Ivo - Pantůček, Roman
PY  - 2001
TI  - Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Macrococcus
KW  - classification
KW  - Llama
KW  - Nucleic Acid Hybridization
KW  - Pigments/metabolism;16S Ribosomal RNA
N2  - Macrococci are gram positive,catalase and oxidase positive cocci inhabiting skin of horses and ponnies. Their pathogenicity is unknown. The genus Macrococcus was earmarked from the genus Staphylococcus and described in 1998. Eight strains isolated from the skin of Lama glama breeded in the ZOO in Brno were described and characterized in this work. All strains studied were gram positive and catalase and oxidase positive, mostly orange-pigmented cocci. Their biochemical traits were in full agreement with the description of the genus Macrococcus. They differ from all hitherto described macrococci by their positive nitrate reduction,negative aesculin and DNA hydrolyses and negative glycerol acidification. The GC content of genomic DNAs were 45.1 - 52.2 %. Following molecular-genetic methods were done to clarify their taxonomic position: ribotyping,macrorestriction analysis and 16S rRNA gene sequencing. All phenotype and genotype analysis results obtained divided studied strains into two homegeneous groups and confirmed that they represent strains of the genus Macrococcus. Moreover,all strains were clearly dictiguished from all hitherto described macrococcal species. Our results imply that they represent two new species of the genus Macrococcus. This suggestion will be confirmed by DNA-DNA hybridisations. All strains studied were deposited in the Czech Collection of Microorganisms as Macrococcus sp. CCM 4808, CCM 4815.
ER  -

DOŠKAŘOVÁ, Sylva, Ivo SEDLÁČEK and Roman PANTŮČEK. \textit{Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas}. 2001.

Detailed Information on Publication Record