DOŠKAŘOVÁ, Sylva, Ivo SEDLÁČEK and Roman PANTŮČEK. Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas. 2001.
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Basic information
Original name Biochemical and molecular taxonomy of gram-positive cocci isolated from skin of llamas
Authors DOŠKAŘOVÁ, Sylva (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, guarantor) and Roman PANTŮČEK (203 Czech Republic).
Edition 2001.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/02:00007081
Organization unit Faculty of Science
Keywords in English Bacterial Typing Techniques; DNA; Macrococcus; classification; Llama; Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA
Tags 16S Ribosomal RNA, Bacterial Typing Techniques, CLASSIFICATION, DNA, Llama, Macrococcus, Nucleic Acid Hybridization, Pigments/metabolism
Changed by Changed by: prof. RNDr. Ivo Sedláček, CSc., učo 866. Changed: 31/3/2010 11:44.
Abstract
Macrococci are gram positive,catalase and oxidase positive cocci inhabiting skin of horses and ponnies. Their pathogenicity is unknown. The genus Macrococcus was earmarked from the genus Staphylococcus and described in 1998. Eight strains isolated from the skin of Lama glama breeded in the ZOO in Brno were described and characterized in this work. All strains studied were gram positive and catalase and oxidase positive, mostly orange-pigmented cocci. Their biochemical traits were in full agreement with the description of the genus Macrococcus. They differ from all hitherto described macrococci by their positive nitrate reduction,negative aesculin and DNA hydrolyses and negative glycerol acidification. The GC content of genomic DNAs were 45.1 - 52.2 %. Following molecular-genetic methods were done to clarify their taxonomic position: ribotyping,macrorestriction analysis and 16S rRNA gene sequencing. All phenotype and genotype analysis results obtained divided studied strains into two homegeneous groups and confirmed that they represent strains of the genus Macrococcus. Moreover,all strains were clearly dictiguished from all hitherto described macrococcal species. Our results imply that they represent two new species of the genus Macrococcus. This suggestion will be confirmed by DNA-DNA hybridisations. All strains studied were deposited in the Czech Collection of Microorganisms as Macrococcus sp. CCM 4808, CCM 4815.
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MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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