D 2002

Discrimination of Armillaria species based on molecular biological methods and HPLC.

LOCHMAN, Jan, Omar ŠERÝ, Vladimír MIKEŠ and Libor JANKOVSKÝ

Basic information

Original name

Discrimination of Armillaria species based on molecular biological methods and HPLC.

Name in Czech

Diskriminace Armillaria metodami mol. biologie a HPLC

Authors

LOCHMAN, Jan (203 Czech Republic, guarantor), Omar ŠERÝ (203 Czech Republic), Vladimír MIKEŠ (203 Czech Republic) and Libor JANKOVSKÝ (203 Czech Republic)

Edition

Kusadasi, Turkey, Book of Abstracts of 7th Meeting of WG, p. 111-111, 2002

Publisher

IOBC, OILB

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Turkey

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00007098

Organization unit

Faculty of Science

Keywords in English

Armillaria; PCR; discrimination; HPLC

Tags

Změněno: 10/2/2005 09:28, prof. RNDr. Vladimír Mikeš, CSc.

Abstract

ORIG CZ

V originále

The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens). We have used the internal transcribed spacer (ITS), which is highly polymorphic and provides useful tool for taxonomic studies thanks to highly conserved sequences of these region. The resolution achieved on the HPLC system was comparable or better than that achieved by electrophoresis and HPLC has better performance in quantitation. Moreover, the restriction analysis of the isolates using HPLC allowed to clearly differentiate some heterozygots from homozygots.

In Czech

Diskriminace Armillaria metodami mol. biologie a HPLC

Links

MSM 143100005, plan (intention)
Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
NF5991, research and development project
Name: Genetika závislostí: zjišťování vztahu polymorfizmů kandidátních genů k alkoholizmu
Investor: Ministry of Health of the CR, Genetic study of alcohol dependence : detection of the association between candidate gene polymorphisms and alcoholism.
NF6520, research and development project
Name: Klinické a molekulárně-biologické koreláty hyperkinetické poruchy v dětském věku
Investor: Ministry of Health of the CR, Clinical and molecular-biological correlates of attention deficit hyperactivity disorder in children