Detailed Information on Publication Record
2002
Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy
WESIERSKA-GADEK, Jozefa, Daniela SCHLOFFER, Vladimír KOTALA and Marcel HORKÝBasic information
Original name
Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy
Authors
WESIERSKA-GADEK, Jozefa (40 Austria, guarantor), Daniela SCHLOFFER (40 Austria), Vladimír KOTALA (203 Czech Republic) and Marcel HORKÝ (203 Czech Republic)
Edition
International Journal of Cancer, USA, Wiley InterScience, 2002, 0020-7136
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.056
RIV identification code
RIV/00216224:14110/02:00007250
Organization unit
Faculty of Medicine
UT WoS
000177622600004
Keywords in English
apoptosis; nucleolar segregation; E6; cisplatin; PARP-1
Tags
Změněno: 16/1/2003 16:13, Mgr. Vladimír Kotala, Ph.D.
Abstract
V originále
We previously reported that therapy of human cervical carcinoma HeLa cells with CP induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. We raised the question of whether p53 can be reactivated by chemotherapy in HeLa cells despite the presence of HPV-encoded E6 activity. Since the reduced stability of wild-type p53 protein in HeLa cells is a consequence of its enhanced ubiquitination by virally encoded E6 protein, resulting in its accelerated degradation, we checked the cellular level of E6 during CP therapy. Six hours after application of CP, E6 protein expression was markedly reduced. This coincided with the increase of cellular p53 and preceded the nucleolar accumulation of p53 protein, indicating that repression of virally coded E6 protein by CP contributes to the restoration of p53 expression.
Links
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