| UMLAUF, Jozef, Šárka KUCHTÍČKOVÁ, B. SLANÁ and Vladimír KOTALA. APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS. Biomarkers and Enviroment. Česká republika: CECHTUMA, s.r.o., 2002, vol. 5, No 1, p. 32-34. ISSN 1211-8869. |
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@article{483362,
author = {Umlauf, Jozef and Kuchtíčková, Šárka and Slaná, B. and Kotala, Vladimír},
article_location = {Česká republika},
article_number = {1},
keywords = {Doxorubicin; Apoptosis; Endothelial cells; Cardiac myocytes; Caspase-3; TUNEL assay},
language = {eng},
issn = {1211-8869},
journal = {Biomarkers and Enviroment},
title = {APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS},
volume = {5},
year = {2002}
}
TY - JOUR
ID - 483362
AU - Umlauf, Jozef - Kuchtíčková, Šárka - Slaná, B. - Kotala, Vladimír
PY - 2002
TI - APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS
JF - Biomarkers and Enviroment
VL - 5
IS - 1
SP - 32
EP - 32
PB - CECHTUMA, s.r.o.
SN - 12118869
KW - Doxorubicin
KW - Apoptosis
KW - Endothelial cells
KW - Cardiac myocytes
KW - Caspase-3
KW - TUNEL assay
N2 - The aim of our study was to detect apoptosis of cardiac myocytes and endothelial cells of the left ventricular myocardium in SHR. We used the TUNEL assay and caspase-3 antibodies to assess critical steps in the pathways of active cell death, which involve rapid proteolysis and final DNA fragmentation. The spontaneously hypertensive rats were treated with doxorubucin for 8 weeks. The left ventricular myocardium was harvested after the 1st, 2nd, 3rd, 4th and 8th weeks. The histological examination revealed a rapid onset of nuclear caspase- 3 positivity as well as TUNEL positivity after 7 days of treatment, which continued in all the periods investigated. We also found caspase-3 positive nuclei in cardiac myocytes after the 1st week of doxorubicin treatment. The number of TUNEL positive cardiomyocytes rose after the second week of treatment and peaked after 4 weeks of treatment. Our data show that apoptosis of endothelial cells and cardiac myocytes plays a critical role in the pathogenesis of doxorubicin induced cardiomyopathy.
ER -
UMLAUF, Jozef, Šárka KUCHTÍČKOVÁ, B. SLANÁ and Vladimír KOTALA. APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS. \textit{Biomarkers and Enviroment}. Česká republika: CECHTUMA, s.r.o., 2002, vol.~5, No~1, p.~32-34. ISSN~1211-8869.
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