Electrochemical impedance spectroscopy of polynucleotide
adsorption.

J 2002

Electrochemical impedance spectroscopy of polynucleotide adsorption.

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ and Vladimír VETTERL

Basic information

Original name

Electrochemical impedance spectroscopy of polynucleotide adsorption.

Authors

STRAŠÁK, Luděk (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), Stanislav HASOŇ (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)

Edition

Bioelectrochemistry, The Netherlands, Elsevier, 2002, 1567-5394

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.463

RIV identification code

RIV/00216224:14310/02:00007362

Organization unit

Faculty of Science

Keywords in English

electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly A adsorption; differential capacitance

Abstract

V originále

The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. from the frequency dependence of the impedance of the electrode double layer represented in a complex plane impedance plot (Nyquist plot, Cole-Cole plot), the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded that the desorption of ssDNA (single stranded, denatured) is accompanied by higher dielectric losses than the desorption of dsDNA (double stranded, native). This can be explained by the higher flexibilioty of ssDNA compared to the dsDNA. The capacitance peak of single stranded polyadenylic acid observed at pH 8 around -1.3 V splits at lopw frequencies in two peaks.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

IAA4004002, research and development project

Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces

Cite

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ and Vladimír VETTERL. Electrochemical impedance spectroscopy of polynucleotide adsorption. Bioelectrochemistry. The Netherlands: Elsevier, 2002, vol. 56, 1-2, p. 37-41. ISSN 1567-5394.

@article{483762,
   author = {Strašák, Luděk and Dvořák, Jakub and Hasoň, Stanislav and Vetterl, Vladimír},
   article_location = {The Netherlands},
   article_number = {1-2},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly A adsorption; differential capacitance},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Electrochemical impedance spectroscopy of polynucleotide adsorption.},
   volume = {56},
   year = {2002}
}

TY  - JOUR
ID  - 483762
AU  - Strašák, Luděk - Dvořák, Jakub - Hasoň, Stanislav - Vetterl, Vladimír
PY  - 2002
TI  - Electrochemical impedance spectroscopy of polynucleotide adsorption.
JF  - Bioelectrochemistry
VL  - 56
IS  - 1-2
SP  - 37
EP  - 37
PB  - Elsevier
SN  - 15675394
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - polyadenylic acid adsorption
KW  - poly A adsorption
KW  - differential capacitance
N2  - The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. from the frequency dependence of the impedance of the electrode double layer represented in a complex plane impedance plot (Nyquist plot, Cole-Cole plot), the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded that the desorption of ssDNA (single stranded, denatured) is accompanied by higher dielectric losses than the desorption of dsDNA (double stranded, native). This can be explained by the higher flexibilioty of ssDNA compared to the dsDNA. The capacitance peak of single stranded polyadenylic acid observed at pH 8 around -1.3 V splits at lopw frequencies in two peaks.
ER  -

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ and Vladimír VETTERL. Electrochemical impedance spectroscopy of polynucleotide adsorption. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2002, vol.~56, 1-2, p.~37-41. ISSN~1567-5394.

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