Detailed Information on Publication Record
2002
Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERLBasic information
Original name
Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
Authors
HASOŇ, Stanislav (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)
Edition
Critical Reviews in Analytical Chemistry, Boca Raton (Florida, USA), CRC Press, 2002, 1040-8347
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10610 Biophysics
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.074
RIV identification code
RIV/00216224:14310/02:00007363
Organization unit
Faculty of Science
Keywords in English
electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;
Tags
Tags
International impact, Reviewed
Změněno: 30/6/2008 14:16, prof. RNDr. Vladimír Vetterl, DrSc.
Abstract
V originále
Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.
Links
GV204/97/K084, research and development project |
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IAA4004002, research and development project |
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