Impedance analysis of DNA and DNA-drug interactions on thin
mercury film electrodes.

J 2002

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL

Basic information

Original name

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

Authors

HASOŇ, Stanislav (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)

Edition

Critical Reviews in Analytical Chemistry, Boca Raton (Florida, USA), CRC Press, 2002, 1040-8347

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.074

RIV identification code

RIV/00216224:14310/02:00007363

Organization unit

Faculty of Science

Keywords in English

electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;

Abstract

V originále

Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

IAA4004002, research and development project

Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces

Citovat

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. Critical Reviews in Analytical Chemistry. Boca Raton (Florida, USA): CRC Press, 2002, vol. 32, No 2, p. 167-179. ISSN 1040-8347.

@article{483763,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   article_location = {Boca Raton (Florida, USA)},
   article_number = {2},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;},
   language = {eng},
   issn = {1040-8347},
   journal = {Critical Reviews in Analytical Chemistry},
   title = {Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.},
   volume = {32},
   year = {2002}
}

TY  - JOUR
ID  - 483763
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2002
TI  - Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
JF  - Critical Reviews in Analytical Chemistry
VL  - 32
IS  - 2
SP  - 167
EP  - 167
PB  - CRC Press
SN  - 10408347
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - echinomycin
KW  - DNA-echinomycin interaction
KW  - mercury film electrodes;
N2  - Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.
ER  -

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. \textit{Critical Reviews in Analytical Chemistry}. Boca Raton (Florida, USA): CRC Press, 2002, vol.~32, No~2, p.~167-179. ISSN~1040-8347.

Detailed Information on Publication Record