Detailed Information on Publication Record
2003
Inhibition study of rhodanese by means of electrophoretically mediated microanalysis
NOVÁKOVÁ, Soňa, Magdalena TELNAROVÁ and Zdeněk GLATZBasic information
Original name
Inhibition study of rhodanese by means of electrophoretically mediated microanalysis
Authors
NOVÁKOVÁ, Soňa (203 Czech Republic), Magdalena TELNAROVÁ (203 Czech Republic) and Zdeněk GLATZ (203 Czech Republic, guarantor)
Edition
Journal of Chromatography A, Netherlands, Elsevier, 2003, 0021-9606
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.950
RIV identification code
RIV/00216224:14310/03:00007945
Organization unit
Faculty of Science
UT WoS
000181647200021
Keywords in English
Capillary electrophoresis; Rhodenase; EMMA; Inhibition
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of the bovine liver rhodanese by 2-oxoglutarate. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. The estimated value of KI for 2-oxoglutarate was 3.62 x 10-4 ą 1.43 x 10-4 M with respect to cyanide and 1.40 x 10-3 ą 1.60 x 10-4 M with respect to thiosulfate. Moreover the type of inhibition was also evaluated. The findings of 2-oxogluatarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data.
Links
| GA203/03/1125, research and development project |
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