D 2001

Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL

Basic information

Original name

Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).

Authors

HASOŇ, Stanislav (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)

Edition

Prague, Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001, p. 42-42, 2001

Publisher

US-CZ Workshop on Electrochemical Sensors Prague

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14310/01:00007523

Organization unit

Faculty of Science

ISBN

80-86238-15-6

Keywords in English

DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes;
Changed: 10/2/2003 17:33, prof. RNDr. Vladimír Vetterl, DrSc.

Abstract

V originále

The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
IAA4004002, research and development project
Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů
Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces