2001
Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERLBasic information
Original name
Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
Authors
HASOŇ, Stanislav (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)
Edition
Prague, Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001, p. 42-42, 2001
Publisher
US-CZ Workshop on Electrochemical Sensors Prague
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
10610 Biophysics
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/00216224:14310/01:00007523
Organization unit
Faculty of Science
ISBN
80-86238-15-6
Keywords in English
DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes;
Tags
Changed: 10/2/2003 17:33, prof. RNDr. Vladimír Vetterl, DrSc.
Abstract
V originále
The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.
Links
GV204/97/K084, research and development project |
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IAA4004002, research and development project |
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