ŘEPKOVÁ, Jana, Pavel LÍZAL, Zdeňka KYJOVSKÁ, Petr RYŠÁVKA and Sylvie HLAVÁČOVÁ. Výsledky a zhodnocení aplikace DNA markerů při genetickém mapování u Arabidopsis thaliana (Results and evaluation of DNA markers utilization in genetic mapping of Arabidopsis thaliana). In Využití molekulárních markerů v biologii, šlechtění a uchování genových zdrojů rostlin. Šumperk: Agritec,výzkum, šlechtění a služby, s.r.o. Šumperk, 2002, p. 147;154, 8 pp.
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Basic information
Original name Výsledky a zhodnocení aplikace DNA markerů při genetickém mapování u Arabidopsis thaliana
Name (in English) Results and evaluation of DNA markers utilization in genetic mapping of Arabidopsis thaliana
Authors ŘEPKOVÁ, Jana (203 Czech Republic, guarantor), Pavel LÍZAL (203 Czech Republic), Zdeňka KYJOVSKÁ (203 Czech Republic), Petr RYŠÁVKA (203 Czech Republic) and Sylvie HLAVÁČOVÁ (203 Czech Republic).
Edition Šumperk, Využití molekulárních markerů v biologii, šlechtění a uchování genových zdrojů rostlin, p. 147;154, 8 pp. 2002.
Publisher Agritec,výzkum, šlechtění a služby, s.r.o. Šumperk
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/02:00007550
Organization unit Faculty of Science
Keywords in English CAPS markers; genetic mapping; microsatellites; SSR markers
Tags CAPS markers, genetic mapping, microsatellites, SSR markers
Changed by Changed by: prof. RNDr. Jana Řepková, CSc., učo 530. Changed: 28/5/2003 12:47.
Abstract
Seven different mutations with defects in immature seed development, one in trichome structure and one in flowering-time have been identified during screening of T-DNA collection of Arabidopsis thaliana. The mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. The second group of analysed mutations were those obtained by classical mutagenesis, five of them in flowering-time, three morphological ones and three with chlorophyll defects. In four tested mutations in flowering-time dominant pattern of inheritance were confirmed and the others were recessive. Codominant SSR and CAPS markers were used for molecular mapping of all identified mutations. Methods of 16 SSR and 9 CAPS markers were optimised. Altogether 20 mutant genes, recessive, dominant and with embryonic lethality were localised in linkage groups of A. thaliana.
Abstract (in English)
Seven different mutations with defects in immature seed development, one in trichome structure and one in flowering-time have been identified during screening of T-DNA collection of Arabidopsis thaliana. The mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. The second group of analysed mutations were those obtained by classical mutagenesis, five of them in flowering-time, three morphological ones and three with chlorophyll defects. In four tested mutations in flowering-time dominant pattern of inheritance were confirmed and the others were recessive. Codominant SSR and CAPS markers were used for molecular mapping of all identified mutations. Methods of 16 SSR and 9 CAPS markers were optimised. Altogether 20 mutant genes, recessive, dominant and with embryonic lethality were localised in linkage groups of A. thaliana.
Links
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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