Immunosensor for the measurement of human serum albumin in
urine based on the Spreeta surface plasmon resonance sensor

J 2003

Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor

NAVRÁTILOVÁ, Iva and Petr SKLÁDAL

Basic information

Original name

Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor

Authors

NAVRÁTILOVÁ, Iva (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Supramolecular Chemistry, USA, Taylor & Francis, 2003, 1061-0278

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.090

RIV identification code

RIV/00216224:14310/03:00008617

Organization unit

Faculty of Science

UT WoS

000181576300005

Keywords in English

Immunosensor;Human serum albumin;Spreeta

Abstract

V originále

The application of SPR for the measurement of concentration of human serum albumin (HSA) in urine was studied using the compact integrated SPR sensing system Spreeta. HSA was immobilized via cystamine and glutaraldehyde onto the gold sensing area and a competitive assay for HSA was developed using limited amount of the monoclonal antibody AL-01 in solution. Measurements were carried out in the flow-through mode and the interaction between immobilized HSA and antibody was observed in real time. To obtain reproducible results, different conditions of the measurement (method of immobilization of HSA, data evaluation, concentration of antibody, regeneration procedure) were tested. The calibration curve for clinically relevant concentrations of HSA in urine samples was constructed using 300-times diluted antibody in the form of ascites fluid. The measuring range was between 0.1 and 5 mg/l of HSA, the sensing surface was successfully regenerated and suitable for more than 20 assays. The developed method was tested on real samples of urine; to overcome the non-specific adsorption of urine components, the differential approach was adopted and the measured signal was corrected by subtraction of the response observed in the absence of the antibody.

Links

OC 518.30, research and development project

Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors

Citovat

NAVRÁTILOVÁ, Iva and Petr SKLÁDAL. Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor. Supramolecular Chemistry. USA: Taylor & Francis, 2003, vol. 15, No 2, p. 109-115. ISSN 1061-0278.

@article{485167,
   author = {Navrátilová, Iva and Skládal, Petr},
   article_location = {USA},
   article_number = {2},
   keywords = {Immunosensor;Human serum albumin;Spreeta},
   language = {eng},
   issn = {1061-0278},
   journal = {Supramolecular Chemistry},
   title = {Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor},
   volume = {15},
   year = {2003}
}

TY  - JOUR
ID  - 485167
AU  - Navrátilová, Iva - Skládal, Petr
PY  - 2003
TI  - Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor
JF  - Supramolecular Chemistry
VL  - 15
IS  - 2
SP  - 109-115
EP  - 109-115
PB  - Taylor & Francis
SN  - 10610278
KW  - Immunosensor;Human serum albumin;Spreeta
N2  - The application of SPR for the measurement of concentration of human serum albumin (HSA) in urine was studied using the compact integrated SPR sensing system Spreeta. HSA was immobilized via cystamine and glutaraldehyde onto the gold sensing area and a competitive assay for HSA was developed using limited amount of the monoclonal antibody AL-01 in solution. Measurements were carried out in the flow-through mode and the interaction between immobilized HSA and antibody was observed in real time. To obtain reproducible results, different conditions of the measurement (method of immobilization of HSA, data evaluation, concentration of antibody, regeneration procedure) were tested. The calibration curve for clinically relevant concentrations of HSA in urine samples was constructed using 300-times diluted antibody in the form of ascites fluid. The measuring range was between 0.1 and 5 mg/l of HSA, the sensing surface was successfully regenerated and suitable for more than 20 assays. The developed method was tested on real samples of urine; to overcome the non-specific adsorption of urine components, the differential approach was adopted and the measured signal was corrected by subtraction of the response observed in the absence of the antibody.
ER  -

NAVRÁTILOVÁ, Iva and Petr SKLÁDAL. Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor. \textit{Supramolecular Chemistry}. USA: Taylor \&{} Francis, 2003, vol.~15, No~2, p.~109-115. ISSN~1061-0278.

Detailed Information on Publication Record