HORKÝ, Marcel, Martin SMRČKA, Filip OTEVŘEL, Šárka KUCHTÍČKOVÁ, Vilém JURÁŇ, M. DUBA and Jan MUŽÍK. Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury. Physiological Research. Praha: Academy of Sciences of the Czech Rep., xxx, xx, p. xxx, 5-in print. ISSN 0862-8408. 2003.
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Basic information
Original name Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury
Authors HORKÝ, Marcel (203 Czech Republic, guarantor), Martin SMRČKA (203 Czech Republic), Filip OTEVŘEL (203 Czech Republic), Šárka KUCHTÍČKOVÁ (203 Czech Republic), Vilém JURÁŇ (203 Czech Republic), M. DUBA (203 Czech Republic) and Jan MUŽÍK (203 Czech Republic).
Edition Physiological Research, Praha, Academy of Sciences of the Czech Rep. 2003, 0862-8408.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.939
RIV identification code RIV/00216224:14110/03:00008625
Organization unit Faculty of Medicine
Keywords in English apoptosis; caspase-3; nucleoli; MADD
Tags apoptosis, caspase-3, MADD, nucleoli
Changed by Changed by: prof. MUDr. Jiří Vácha, DrSc., učo 1327. Changed: 14/3/2003 11:28.
Abstract
We focused on histochemical detection of distribution of NOR (argyrophylic nucleolar proteins) reflecting nucleolar integrity, immunohistochemical detection of MADD (mitogen activated death domain), a protein accumulated in nucleoli upon stimulation by ischemia and active form of caspase-3, a universal proteolytic enzyme of apoptosis. The terminal deoxynucleotidyl- transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) proved the presence of in situ DNA fragmentation. We used the model of transient focal cerebral ischemia in rats. The period of ischemia lasted 15, 30, 60 and 120 minutes followed by 48 hrs of reperfusion. We examined the frontal lobe of the ipsilateral hemisphere. We found disintegrated nucleoli in all investigated periods of ischemia in cortical as well as subcortical neurons whereas the neurons of intact animals showed compact nucleoli with a few satellites. Nuclear positivity for MADD was apparent after 15 min. in neocortex and peaked after 30 min. of ischemia. On the other hand, the subcortical neurons showed nuclear positivity for MADD after 60 and 120 minutes. The intact brain tissue showed negative nuclei with only a slight cytoplasmic staining. The immunohistochemical reaction for active caspase 3 was apparent after 30 minutes onwards predominantly in cortex. The controls were caspase 3 negative. The TUNEL staining was remarkable after 60 and 120 minutes. We detected a rapid onset of mucleolar segregation in cortical and subcortical neurons damaged by ischemia (15 min) reperfusion injury which coincides with a nuclear/nucleolar accumulation of MADD, a stress activated neuron specific death domain. Active caspase 3 and TUNEL positivity were found after a prolonged ischemia (30, 60 and 120 min.)
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MSM 141100002, plan (intention)Name: Molekulární patofyziologie multigenních chorob
Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases
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