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@article{485603,
author = {Amrichová, Jana and Lukášová, Emilie and Kozubek, Stanislav and Kozubek, Michal},
article_number = {1},
keywords = {nuclear architecture; telomere; centromere; chromosome territory; chromatin structure; interphase chromosome; human lymphocytes; telomere association; telomere tethering},
language = {eng},
issn = {0014-4827},
journal = {Experimental Cell Research},
title = {Nuclear and territorial topography of chromosome telomeres in human lymphocytes},
url = {http://www.ncbi.nlm.nih.gov:80/entrez/utils/fref.fcgi?http://linkinghub.elsevier.com/retrieve/pii/S0014482703002088},
volume = {289},
year = {2003}
}
TY - JOUR
ID - 485603
AU - Amrichová, Jana - Lukášová, Emilie - Kozubek, Stanislav - Kozubek, Michal
PY - 2003
TI - Nuclear and territorial topography of chromosome telomeres in human lymphocytes
JF - Experimental Cell Research
VL - 289
IS - 1
SP - 11-26
EP - 11-26
PB - Elsevier Science, USA
SN - 00144827
KW - nuclear architecture
KW - telomere
KW - centromere
KW - chromosome territory
KW - chromatin structure
KW - interphase chromosome
KW - human lymphocytes
KW - telomere association
KW - telomere tethering
UR - http://www.ncbi.nlm.nih.gov:80/entrez/utils/fref.fcgi?http://linkinghub.elsevier.com/retrieve/pii/S0014482703002088
N2 - Nuclear and territorial positioning of p- and q- telomeres and centromeres of chromosomes 3, 8, 9, 13 and 19 were studied by repeated FISH, high-resolution cytometry and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is non-randomly folded. Telomeres are in average much nearer to the center of the cell nucleus than centromeres; q-telomeres were found in average more centrally localized as compared with p-telomeres. Consequently, we directly showed that chromosome territories in the cell nucleus are (i) polar and (ii) partially oriented in cell nuclei. The distributions of genetic elements relative to chromosome territories (territorial distributions) can be either narrower or broader than their nuclear distributions, which reflects the degree of adhesion of an element to the territory or to the nucleus. We found no tethering of heterologous telomeres of chromosomes 8, 9, and 19. In contrary, both pairs of homologous telomeres of chromosome 19 (but not in other chromosomes) are tethered (associated) very frequently.
ER -
AMRICHOVÁ, Jana, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK and Michal KOZUBEK. Nuclear and territorial topography of chromosome telomeres in human lymphocytes. \textit{Experimental Cell Research}. Elsevier Science, USA, 2003, vol.~289, No~1, p.~11-26. ISSN~0014-4827.
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