NAGATA, Yuji, Zbyněk PROKOP, Soňa MARVANOVÁ, Jana SÝKOROVÁ, Marta MONINCOVÁ, Masataka TSUDA and Jiří DAMBORSKÝ. Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB. Applied and Environmental Microbiology. 2003, vol. 69, No 4, p. 2349-2355. ISSN 1098-5336. |
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@article{485836, author = {Nagata, Yuji and Prokop, Zbyněk and Marvanová, Soňa and Sýkorová, Jana and Monincová, Marta and Tsuda, Masataka and Damborský, Jiří}, article_number = {4}, keywords = {MUTANT; EVOLUTION; MYCOBACTERIUM; DEHALOGENATION; BIODEGRADATION}, language = {eng}, issn = {1098-5336}, journal = {Applied and Environmental Microbiology}, title = {Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB}, url = {http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem03a.html}, volume = {69}, year = {2003} }
TY - JOUR ID - 485836 AU - Nagata, Yuji - Prokop, Zbyněk - Marvanová, Soňa - Sýkorová, Jana - Monincová, Marta - Tsuda, Masataka - Damborský, Jiří PY - 2003 TI - Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB JF - Applied and Environmental Microbiology VL - 69 IS - 4 SP - 2349-2355 EP - 2349-2355 SN - 10985336 KW - MUTANT KW - EVOLUTION KW - MYCOBACTERIUM KW - DEHALOGENATION KW - BIODEGRADATION UR - http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem03a.html N2 - The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 and uncovered that six out of nineteen amino acid residues which form an active site and entrance tunnel are different between LinB and Rv2579. To characterize an effect of replacements of these six amino acid residues, mutations were introduced cumulatively into the six amino acid residues of LinB. The six-fold mutant which is supposed to have an active site of Rv2579 showed haloalkane dehalogenase activity with tested haloalkanes, confirming that Rv2579 is a member of haloalkane dehalogenase protein family. ER -
NAGATA, Yuji, Zbyněk PROKOP, Soňa MARVANOVÁ, Jana SÝKOROVÁ, Marta MONINCOVÁ, Masataka TSUDA and Jiří DAMBORSKÝ. Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB. \textit{Applied and Environmental Microbiology}. 2003, vol.~69, No~4, p.~2349-2355. ISSN~1098-5336.
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