KAŠNÁ, Andrea and Petr SKLÁDAL. The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids. International Journal of Environmental and Analytical Chemistry. Velká Británie: Taylor & Francis, 2002, vol. 83, No 2, p. 101-109. ISSN 0306-7319.
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Basic information
Original name The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.
Authors KAŠNÁ, Andrea (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor).
Edition International Journal of Environmental and Analytical Chemistry, Velká Británie, Taylor & Francis, 2002, 0306-7319.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.765
RIV identification code RIV/00216224:14310/02:00007720
Organization unit Faculty of Science
Keywords in English Electrochemical immunosensor; Multichannel biosensor; Herbicides; Phenoxyalkanoic acids
Tags Electrochemical immunosensor, Herbicides, Multichannel biosensor, Phenoxyalkanoic acids
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 28/4/2003 15:50.
Abstract
The electrochemical immunosensor combining three monoclonal antibodies on the same measuring element produced by screen-printing was constructed for the semiquantitative group-specific detection of herbicides belonging to phenoxyalkanoic acids. The combination of different monoclonal antibodies covalently immobilized on three working electrodes and one additional electrode containing immobilized albumin provided a more reliable detection of phenoxyalkanoic acids. Peroxidase-2,4-dichlorophenoxyacetic acid conjugate was used as a tracer suitable for all the antibodies employed in a competitive immunoassay. Several herbicides were used to characterize the immunosensor. The developed immunosensor was tested on samples of surface water spiked with different herbicides and binary mixtures of herbicides. The proposed approach seems feasible for rapid screening of large series of samples for the presence of herbicides.
Links
GA525/97/0924, research and development projectName: Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě
Investor: Czech Science Foundation, Trace analysis of toxic PCB and pesticides using detection and separation methods based on antibody specifity
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