Differential roles of Fos and Jun proteins in regulation of
differentiation and apoptosis of v-myb-transformed monoblasts

D 2003

Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA, Jan ŠMARDA et. al.

Basic information

Original name

Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts

Authors

ZAHRADNÍČKOVÁ, Eva (203 Czech Republic), Sabina ŠEVČÍKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic), Lukáš KUBALA (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor)

Edition

Silverthorne, USA, Antibody-based therapeutics for cancer, p. 62-62, 2003

Publisher

Keystone Symposia

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Canada

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008028

Organization unit

Faculty of Science

ISBN

970-262-1230

Keywords in English

Fos; Jun; apoptosis; differentiation; monoblast

Abstract

V originále

The line of v-myb-transformed chicken monoblasts BM2 constitutively express v-myb oncogene of AMV. v-Myb causes differentiation block, high proliferation rate and resistance to apoptosis similarly as in v-myb-transformed myeloid precursor primary cells. Differentiation capability of BM2 cells to macrophage-like cells can resume by phorbol esters that activate PKC and transcription from promoters containing TRE. The fact that transcription factor AP-1 binds TRE prompted us to investigate the role of individual components of AP-1 - the Jun and Fos proteins in regulation of differentiation, proliferation and apoptosis of BM2 cells. We ectopically expressed c-Jun, c-Fos, v-Jun and v-Fos proteins in BM2 cells and compared their effects. We found that c-Fos but not c-Jun protein was capable to induce apoptosis of BM2 cells. Interestingly, transforming variant of the Fos protein (v-Fos) lost capacity of its cellular precursor to cause apoptosis of BM2 cells. On contrary, c-Jun but not c-Fos protein induced differentiation of BM2 cells to adherent macrophage-like cells. Both Fos and Jun proteins down-regulated proliferation of BM2 cells suggesting that individual components of AP-1 transcription factor possess distinct roles in determination of cell afte. Therefore, the presence of both Jun and Fos proteins in the cell seems to be optimal for tumor-suppressive processes.

Links

GA301/01/0040, research and development project

Name: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Czech Science Foundation, The Role of Fos and Jun proteins in RA-driven monocytic differentiation

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA and Jan ŠMARDA. Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts. In Antibody-based therapeutics for cancer. Silverthorne, USA: Keystone Symposia, 2003, p. 62. ISBN 970-262-1230.

@inproceedings{486513,
   author = {Zahradníčková, Eva and Ševčíková, Sabina and Souček, Karel and Kubala, Lukáš and Šmarda, Jan},
   address = {Silverthorne, USA},
   booktitle = {Antibody-based therapeutics for cancer},
   keywords = {Fos; Jun; apoptosis; differentiation; monoblast},
   language = {eng},
   location = {Silverthorne, USA},
   isbn = {970-262-1230},
   pages = {62-62},
   publisher = {Keystone Symposia},
   title = {Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts},
   year = {2003}
}

TY  - JOUR
ID  - 486513
AU  - Zahradníčková, Eva - Ševčíková, Sabina - Souček, Karel - Kubala, Lukáš - Šmarda, Jan
PY  - 2003
TI  - Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts
PB  - Keystone Symposia
CY  - Silverthorne, USA
SN  - 9702621230
KW  - Fos
KW  - Jun
KW  - apoptosis
KW  - differentiation
KW  - monoblast
N2  - The line of v-myb-transformed chicken monoblasts BM2 constitutively express v-myb oncogene of AMV. v-Myb causes differentiation block, high proliferation rate and resistance to apoptosis similarly as in v-myb-transformed myeloid precursor primary cells. Differentiation capability of BM2 cells to macrophage-like cells can resume by phorbol esters that activate PKC and transcription from promoters containing TRE. The fact that transcription factor AP-1 binds TRE prompted us to investigate the role of individual components of AP-1 - the Jun and Fos proteins in regulation of differentiation, proliferation and apoptosis of BM2 cells. We ectopically expressed c-Jun, c-Fos, v-Jun and v-Fos proteins in BM2 cells and compared their effects. We found that c-Fos but not c-Jun protein was capable to induce apoptosis of BM2 cells. Interestingly, transforming variant of the Fos protein (v-Fos) lost capacity of its cellular precursor to cause apoptosis of BM2 cells. On contrary, c-Jun but not c-Fos protein induced differentiation of BM2 cells to adherent macrophage-like cells. Both Fos and Jun proteins down-regulated proliferation of BM2 cells suggesting that individual components of AP-1 transcription factor possess distinct roles in determination of cell afte. Therefore, the presence of both Jun and Fos proteins in the cell seems to be optimal for tumor-suppressive processes.
ER  -

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA and Jan ŠMARDA. Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts. In \textit{Antibody-based therapeutics for cancer}. Silverthorne, USA: Keystone Symposia, 2003, p.~62. ISBN~970-262-1230.

Detailed Information on Publication Record