Detection of 13q abnormalities in multiple myeloma using
immunomagnetically selected plasma cells.

J 2002

Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells.

FIŠEROVÁ, Anežka, Roman HÁJEK, V. HOLUBOVÁ, Tomáš BÜCHLER, J. SOBOTKA et. al.

Basic information

Original name

Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells.

Authors

FIŠEROVÁ, Anežka (203 Czech Republic), Roman HÁJEK (203 Czech Republic, guarantor), V. HOLUBOVÁ (203 Czech Republic), Tomáš BÜCHLER (703 Slovakia), J. SOBOTKA (203 Czech Republic), Lucie KOVÁŘOVÁ (203 Czech Republic), Romana MUSILOVÁ (203 Czech Republic), Ludmila BOURKOVÁ (203 Czech Republic), Alena BULIKOVÁ (203 Czech Republic), Iveta MARESCHOVÁ (203 Czech Republic), Zuzana JANÁČKOVÁ (203 Czech Republic), Pavlína VÁŇOVÁ (203 Czech Republic), P. KUGLÍK (203 Czech Republic), Jiří VORLÍČEK (203 Czech Republic) and Miroslav PENKA (203 Czech Republic)

Edition

Neoplasma, 2002, 0028-2685

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.679

RIV identification code

RIV/00216224:14110/02:00007784

Organization unit

Faculty of Medicine

Keywords in English

multiple myeloma; magnetic-activated cell separation; interphase fluorescence in situ hybridization; Sydecan-1; 13q14 deletion

Abstract

V originále

Accurate prognostic evaluation of patients with multiple myeloma (MM) is required for their stratification for more adequate therapy. Chromosomal G-banding and interphase fluorescence in situ hybridization (FISH) on cell-nonspecific samples and on myeloma cells selected by magnetic-activated cell separation (MACS) were used to study 13 samples from 12 multiple myeloma (MM) patients. Bone marrow (BM) samples were analysed using three approaches. Standard mitotic samples were prepared and analysed after G-banding. Interphase FISH was performed to detect the 13q14 deletion in unselected BM cells. In parallel, myeloma cells were selected from the BM using the CD138-specific antibody. The high-purity myeloma cell suspension was then analysed by interphase FISH for the 13q14 deletion. Magnetic separation yielded enriched myeloma cell suspensions with the mean viability of 98.0% (range: 97.0%-99.0%), and the purity of 97.6% (range: 87.2%-99.2%) as detected morphologically, and 85.2% (range: 44.8%-98.4%) as detected by immunophenotyping for CD138+ cells. Interphase FISH revealed the 13q14.3 deletion in 5 of 13 (38.5%) of cell-nonspecific samples and in 9 of 13 (69.2%) of enriched myeloma cell suspensions. In conclusion, interphase FISH on immunomagnetically selected MM cells increases the detection of the 13q14 deletion in BM samples from the patients with MM.

Links

MSM 141100003, plan (intention)

Name: Zevní prostředí - karcinogeneze - onkologie

Investor: Ministry of Education, Youth and Sports of the CR, Environment - carcinogenesis - oncology

Citovat

FIŠEROVÁ, Anežka, Roman HÁJEK, V. HOLUBOVÁ, Tomáš BÜCHLER, J. SOBOTKA, Lucie KOVÁŘOVÁ, Romana MUSILOVÁ, Ludmila BOURKOVÁ, Alena BULIKOVÁ, Iveta MARESCHOVÁ, Zuzana JANÁČKOVÁ, Pavlína VÁŇOVÁ, P. KUGLÍK, Jiří VORLÍČEK and Miroslav PENKA. Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells. Neoplasma. 2002, 49/2002, No 5, p. 300-306. ISSN 0028-2685.

@article{486671,
   author = {Fišerová, Anežka and Hájek, Roman and Holubová, V. and Büchler, Tomáš and Sobotka, J. and Kovářová, Lucie and Musilová, Romana and Bourková, Ludmila and Buliková, Alena and Mareschová, Iveta and Janáčková, Zuzana and Váňová, Pavlína and Kuglík, P. and Vorlíček, Jiří and Penka, Miroslav},
   article_number = {5},
   keywords = {multiple myeloma; magnetic-activated cell separation; interphase fluorescence in situ hybridization; Sydecan-1; 13q14 deletion},
   language = {eng},
   issn = {0028-2685},
   journal = {Neoplasma},
   title = {Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells.},
   volume = {49/2002},
   year = {2002}
}

TY  - JOUR
ID  - 486671
AU  - Fišerová, Anežka - Hájek, Roman - Holubová, V. - Büchler, Tomáš - Sobotka, J. - Kovářová, Lucie - Musilová, Romana - Bourková, Ludmila - Buliková, Alena - Mareschová, Iveta - Janáčková, Zuzana - Váňová, Pavlína - Kuglík, P. - Vorlíček, Jiří - Penka, Miroslav
PY  - 2002
TI  - Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells.
JF  - Neoplasma
VL  - 49/2002
IS  - 5
SP  - 300-306
EP  - 300-306
SN  - 00282685
KW  - multiple myeloma
KW  - magnetic-activated cell separation
KW  - interphase fluorescence in situ hybridization
KW  - Sydecan-1
KW  - 13q14 deletion
N2  - Accurate prognostic evaluation of patients with multiple myeloma (MM) is required for their stratification for more adequate therapy. Chromosomal G-banding and interphase fluorescence in situ hybridization (FISH) on cell-nonspecific samples and on myeloma cells selected by magnetic-activated cell separation (MACS) were used to study 13 samples from 12 multiple myeloma (MM) patients. Bone marrow (BM) samples were analysed using three approaches. Standard mitotic samples were prepared and analysed after G-banding. Interphase FISH was performed to detect the 13q14 deletion in unselected BM cells. In parallel, myeloma cells were selected from the BM using the CD138-specific antibody. The high-purity myeloma cell suspension was then analysed by interphase FISH for the 13q14 deletion. Magnetic separation yielded enriched myeloma cell suspensions with the mean viability of 98.0% (range: 97.0%-99.0%), and the purity of 97.6% (range: 87.2%-99.2%) as detected morphologically, and 85.2% (range: 44.8%-98.4%) as detected by immunophenotyping for CD138+ cells. Interphase FISH revealed the 13q14.3 deletion in 5 of 13 (38.5%) of cell-nonspecific samples and in 9 of 13 (69.2%) of enriched myeloma cell suspensions. In conclusion, interphase FISH on immunomagnetically selected MM cells increases the detection of the 13q14 deletion in BM samples from the patients with MM.
ER  -

FIŠEROVÁ, Anežka, Roman HÁJEK, V. HOLUBOVÁ, Tomáš BÜCHLER, J. SOBOTKA, Lucie KOVÁŘOVÁ, Romana MUSILOVÁ, Ludmila BOURKOVÁ, Alena BULIKOVÁ, Iveta MARESCHOVÁ, Zuzana JANÁČKOVÁ, Pavlína VÁŇOVÁ, P. KUGLÍK, Jiří VORLÍČEK and Miroslav PENKA. Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells. \textit{Neoplasma}. 2002, 49/2002, No~5, p.~300-306. ISSN~0028-2685.

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