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@article{486689,
author = {Büchler, Tomáš and Hájek, Roman and Bourková, Ludmila and Kovářová, Lucie and Musilová, Romana and Buliková, Alena and Doubek, M. and Svobodník, A. and Mareschová, Iveta and Váňová, Pavlína and Tůzová, Eva and Vidláková, Petra and Vorlíček, Jiří and Penka, Miroslav},
article_number = {2/2003},
keywords = {dendritic cells; in vitro cell culture; cytokines; immunotherapy},
language = {eng},
issn = {0264-410X},
journal = {Vaccine},
title = {Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.},
volume = {neuveden},
year = {2003}
}
TY - JOUR
ID - 486689
AU - Büchler, Tomáš - Hájek, Roman - Bourková, Ludmila - Kovářová, Lucie - Musilová, Romana - Buliková, Alena - Doubek, M. - Svobodník, A. - Mareschová, Iveta - Váňová, Pavlína - Tůzová, Eva - Vidláková, Petra - Vorlíček, Jiří - Penka, Miroslav
PY - 2003
TI - Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations.
JF - Vaccine
VL - neuveden
IS - 2/2003
SP - 877-882
EP - 877-882
SN - 0264410X
KW - dendritic cells
KW - in vitro cell culture
KW - cytokines
KW - immunotherapy
N2 - Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions. Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers. As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells were cultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-[alpha], Flt-3, CD40L, IFN-[gamma], IL-1[alpha], IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin-12 assay was performed. For PBSC, the yield of DC as determined by CD83+ cell count ranged from 0.6 x 105 to 30.1 x 104 (mean: 9.4 x 104) of DC generated per 1 x 106 of initially plated nucleated cells from apheresis. This yield corresponded to (0.3-19.1) x 105 (mean: 4.3 x 105) per 1 x 106 of CD34+ cells in the apheresis products. For PBMC, the yield was (0.4-24.8) x 104 (mean: 2.4 x 104) of DC generated per 1 x 106 of initially plated mononuclear cells from venous blood. The cultured cells expressed the mature immunophenotype. No significant differences in cell yield or immunophenotype were detected when comparing different cytokine combinations. (C) 2002 Elsevier Science Ltd. All rights reserved.
ER -
BÜCHLER, Tomáš, Roman HÁJEK, Ludmila BOURKOVÁ, Lucie KOVÁŘOVÁ, Romana MUSILOVÁ, Alena BULIKOVÁ, M. DOUBEK, A. SVOBODNÍK, Iveta MARESCHOVÁ, Pavlína VÁŇOVÁ, Eva TŮZOVÁ, Petra VIDLÁKOVÁ, Jiří VORLÍČEK and Miroslav PENKA. Generation of antigen-loaded dendritic cells in a serum-free medium using different cytokine combinations. \textit{Vaccine}. 2003, neuveden, 2/2003, p.~877-882. ISSN~0264-410X.
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