Use of osmium tetroxide-bipyridine reagent in MALDI-TOF MS analysis of peptides including [Gly14]-humanin derivatives

ŠEDO, Ondrej, Klára NOVOTNÁ and Josef HAVEL. Use of osmium tetroxide-bipyridine reagent in MALDI-TOF MS analysis of peptides including [Gly14]-humanin derivatives. In Biologicky aktivní peptidy VIII. Praha: ÚOCHB AV ČR, 2003, p. 45. ISBN 80-86241-18-1.

Basic information

• Original name: Use of osmium tetroxide-bipyridine reagent in MALDI-TOF MS analysis of peptides including [Gly14]-humanin derivatives
• Authors: ŠEDO, Ondrej (203 Czech Republic), Klára NOVOTNÁ (203 Czech Republic) and Josef HAVEL (203 Czech Republic, guarantor).
• Edition: Praha, Biologicky aktivní peptidy VIII. p. 45-45, 2003.
• Publisher: ÚOCHB AV ČR

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Denmark
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/03:00008056
• Organization unit: Faculty of Science
• ISBN: 80-86241-18-1
• Keywords in English: osmium tetroxide; bipyridine; humanin; MALDI-TOF MS; PSD
• Tags: bipyridine, humanin, MALDI-TOF MS, osmium tetroxide, PSD

Abstract

Osmium tetroxide-bipyridine (Os,bipy) is a reagent which has successfully been applied in analysis of DNA and it has been recently proposed as a suitable probe for peptide derivatization because of its ability to form stable complex with tryptophan residues. In this work the reaction of Os,bipy with peptides including humanin derivates was studied using Matrix Assisted Laser Desorption-Ionisation - Time of Flight Mass Spectrometry (MALDI-TOF MS).

Links

• GA305/03/1100, research and development project: Name: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu