Specific determination of cysteine in human urine by capillary
micellar electrokinetic chromatography

J 2003

Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

ŠEVČÍKOVÁ, Petra and Zdeněk GLATZ

Basic information

Original name

Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

Authors

ŠEVČÍKOVÁ, Petra (203 Czech Republic) and Zdeněk GLATZ (203 Czech Republic, guarantor)

Edition

Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2003, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008072

Organization unit

Faculty of Science

UT WoS

000183660100015

Keywords in English

urine; cysteine; MEKC; on-column reaction

Abstract

V originále

This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.

Links

GA203/03/1125, research and development project

Name: Využití kapilární zónové elektroforézy pro studium enzymů

Investor: Czech Science Foundation, Application of capillary zone electrophoresis for the study of enzymes

Citovat

ŠEVČÍKOVÁ, Petra and Zdeněk GLATZ. Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2003, vol. 26, No 8, p. 734-738. ISSN 1615-9306.

@article{487342,
   author = {Ševčíková, Petra and Glatz, Zdeněk},
   article_location = {Weinheim},
   article_number = {8},
   keywords = {urine; cysteine; MEKC; on-column reaction},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography},
   volume = {26},
   year = {2003}
}

TY  - JOUR
ID  - 487342
AU  - Ševčíková, Petra - Glatz, Zdeněk
PY  - 2003
TI  - Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography
JF  - Journal of Separation Science
VL  - 26
IS  - 8
SP  - 734-738
EP  - 734-738
PB  - WILEY-VCH Verlag GmbH & Co.
SN  - 16159306
KW  - urine
KW  - cysteine
KW  - MEKC
KW  - on-column reaction
N2  - This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.
ER  -

ŠEVČÍKOVÁ, Petra and Zdeněk GLATZ. Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2003, vol.~26, No~8, p.~734-738. ISSN~1615-9306.

Detailed Information on Publication Record