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@article{487342,
author = {Ševčíková, Petra and Glatz, Zdeněk},
article_location = {Weinheim},
article_number = {8},
keywords = {urine; cysteine; MEKC; on-column reaction},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography},
volume = {26},
year = {2003}
}
TY - JOUR
ID - 487342
AU - Ševčíková, Petra - Glatz, Zdeněk
PY - 2003
TI - Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography
JF - Journal of Separation Science
VL - 26
IS - 8
SP - 734-738
EP - 734-738
PB - WILEY-VCH Verlag GmbH & Co.
SN - 16159306
KW - urine
KW - cysteine
KW - MEKC
KW - on-column reaction
N2 - This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.
ER -
ŠEVČÍKOVÁ, Petra and Zdeněk GLATZ. Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2003, vol.~26, No~8, p.~734-738. ISSN~1615-9306.
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