Bioluminiscenční, elektroforetická a biochemická
charakteristika nového českého izolátu z rodu Photorhabdus

D 2003

Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus

HYRŠL, Pavel, Milan ČÍŽ, Ivo SEDLÁČEK, Zdenka PÁČOVÁ, Antonín LOJEK et. al.

Basic information

Original name

Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus

Name (in English)

Bioluminescent, electrophoretical and biochemical determination of a new Photorhabdus isolate from Czech Republic

Authors

HYRŠL, Pavel, Milan ČÍŽ, Ivo SEDLÁČEK, Zdenka PÁČOVÁ and Antonín LOJEK

Edition

Brno, Sborník abstraktů Tomáškovy dny 2003, 1 pp. 2003

Publisher

Mikrobiologický ústav LF MU a FN u svaté Anny v Brně

Other information

Type of outcome

Stať ve sborníku

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Změněno: 20/3/2006 15:17, doc. RNDr. Pavel Hyršl, Ph.D.

Abstract

V originále

We isolated symbiotic bacteria from entomopathogenic nematode Heterorhabditis bacteriophora H221 (Pouzdřany). Our isolate CCM 7002 shows clear luminescence and this is characteristic for genus Photorhabdus, the only known terrestrial luminescent bacteria. Thus our isolate was compared with type cultures of Photorhabdus species and subspecies (P. luminescens ssp. luminescens, P. luminescens ssp. laumondii, P. luminescens ssp. akhurstii, P. asymbiotica and P. temperata). All Photorhabdus strains have been shown luminescence (bioluminescence). Luminescence was measured on luminometer (BioOrbit 1251) at 25 and 37 C for two hours. Luminescent signal is rising at 25 C and at the temperature of 37 C is falling or constant. Each tested strain has a typical luminescent signal. The highest value shows always P. temperata. To compare the protein spectrum of studied bacteria species, bacterial proteins were analysed by acrylamide gradient gel electrophoresis (SDS-PAGGE). We found more than 40 protein fractions in the range of 6.5 200 kDa. We demonstrated significant differences between the species and subspecies of genus Photorhabdus. Phenotypic characteristics were based on identification kits (API 20 E, API 20 NE, ENTEROtest 24) supplemented with additional conventional tests. Identification of the CCM 7002 to the species level remains dubious due to low biochemical activity. Mostly related species to CCM 7002 on the basis of phenotyping is P. luminescens species.

Citovat

HYRŠL, Pavel, Milan ČÍŽ, Ivo SEDLÁČEK, Zdenka PÁČOVÁ and Antonín LOJEK. Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus (Bioluminescent, electrophoretical and biochemical determination of a new Photorhabdus isolate from Czech Republic). In Sborník abstraktů Tomáškovy dny 2003. Brno: Mikrobiologický ústav LF MU a FN u svaté Anny v Brně, 2003, 1 pp.

@inproceedings{487385,
   author = {Hyršl, Pavel and Číž, Milan and Sedláček, Ivo and Páčová, Zdenka and Lojek, Antonín},
   address = {Brno},
   booktitle = {Sborník abstraktů Tomáškovy dny 2003},
   location = {Brno},
   publisher = {Mikrobiologický ústav LF MU a FN u svaté Anny v Brně},
   title = {Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus},
   year = {2003}
}

TY  - JOUR
ID  - 487385
AU  - Hyršl, Pavel - Číž, Milan - Sedláček, Ivo - Páčová, Zdenka - Lojek, Antonín
PY  - 2003
TI  - Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus
PB  - Mikrobiologický ústav LF MU a FN u svaté Anny v Brně
CY  - Brno
N2  - We isolated symbiotic bacteria from entomopathogenic nematode Heterorhabditis bacteriophora H221 (Pouzdřany). Our isolate CCM 7002 shows clear luminescence and this is characteristic for genus Photorhabdus, the only known terrestrial luminescent bacteria. Thus our isolate was compared with type cultures of Photorhabdus species and subspecies (P. luminescens ssp. luminescens, P. luminescens ssp. laumondii, P. luminescens ssp. akhurstii, P. asymbiotica and P. temperata). All Photorhabdus strains have been shown luminescence (bioluminescence). Luminescence was measured on luminometer (BioOrbit 1251) at 25 and 37 C for two hours. Luminescent signal is rising at 25 C and at the temperature of 37 C is falling or constant. Each tested strain has a typical luminescent signal. The highest value shows always P. temperata. To compare the protein spectrum of studied bacteria species, bacterial proteins were analysed by acrylamide gradient gel electrophoresis (SDS-PAGGE). We found more than 40 protein fractions in the range of 6.5 200 kDa. We demonstrated significant differences between the species and subspecies of genus Photorhabdus. Phenotypic characteristics were based on identification kits (API 20 E, API 20 NE, ENTEROtest 24) supplemented with additional conventional tests. Identification of the CCM 7002 to the species level remains dubious due to low biochemical activity. Mostly related species to CCM 7002 on the basis of phenotyping is P. luminescens species.
ER  -

HYRŠL, Pavel, Milan ČÍŽ, Ivo SEDLÁČEK, Zdenka PÁČOVÁ and Antonín LOJEK. Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus (Bioluminescent, electrophoretical and biochemical determination of a new Photorhabdus isolate from Czech Republic). In \textit{Sborník abstraktů Tomáškovy dny 2003}. Brno: Mikrobiologický ústav LF MU a FN u svaté Anny v Brně, 2003, 1 pp.

Detailed Information on Publication Record