Proteomic Analysis of Therapeutically Important Bacteriophage
812 by Combination of Electrophoresis and Mass Spectrometry

D 2003

Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL et. al.

Basic information

Original name

Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry

Authors

PREISLER, Jan (203 Czech Republic, guarantor), Zbyněk ZDRÁHAL (203 Czech Republic), Karel KLEPÁRNÍK (203 Czech Republic), František FORET (203 Czech Republic), Patrik VRÁBEL (203 Czech Republic), Zdena MALÁ (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Hana KONEČNÁ (203 Czech Republic) and Pavel KRÁSENSKÝ (203 Czech Republic)

Edition

Montreal, Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics, p. 1152-1152, 2003

Publisher

American Society for Mass Spectrometry

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Canada

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008100

Organization unit

Faculty of Science

Keywords in English

MALDI; staphylococcal bacteriophage; proteome

Abstract

V originále

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally, protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS.

Links

GA203/03/0515, research and development project

Name: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Investor: Czech Science Foundation, Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL, Zdena MALÁ, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ and Pavel KRÁSENSKÝ. Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry. In Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics. Montreal: American Society for Mass Spectrometry, 2003, p. 1152.

@inproceedings{487584,
   author = {Preisler, Jan and Zdráhal, Zbyněk and Klepárník, Karel and Foret, František and Vrábel, Patrik and Malá, Zdena and Doškař, Jiří and Pantůček, Roman and Růžičková, Vladislava and Konečná, Hana and Krásenský, Pavel},
   address = {Montreal},
   booktitle = {Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics},
   keywords = {MALDI; staphylococcal bacteriophage; proteome},
   language = {eng},
   location = {Montreal},
   pages = {1152-1152},
   publisher = {American Society for Mass Spectrometry},
   title = {Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry},
   year = {2003}
}

TY  - JOUR
ID  - 487584
AU  - Preisler, Jan - Zdráhal, Zbyněk - Klepárník, Karel - Foret, František - Vrábel, Patrik - Malá, Zdena - Doškař, Jiří - Pantůček, Roman - Růžičková, Vladislava - Konečná, Hana - Krásenský, Pavel
PY  - 2003
TI  - Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry
PB  - American Society for Mass Spectrometry
CY  - Montreal
KW  - MALDI
KW  - staphylococcal bacteriophage
KW  - proteome
N2  - The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally, protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS.
ER  -

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL, Zdena MALÁ, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ and Pavel KRÁSENSKÝ. Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry. In \textit{Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics}. Montreal: American Society for Mass Spectrometry, 2003, p.~1152.

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