Electrochemical determination of glutathion and  phytochelatins
redox state

D 2003

Electrochemical determination of glutathion and phytochelatins redox state

VACEK, Jan, Libuše TRNKOVÁ, René KIZEK and František JELEN

Basic information

Original name

Electrochemical determination of glutathion and phytochelatins redox state

Authors

VACEK, Jan (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), René KIZEK (203 Czech Republic) and František JELEN (203 Czech Republic)

Edition

1. vyd. Florence, Italy, XVII th International Symposium on Bioelectrochemistry and Bioenergetics, p. 251-251, 2003

Publisher

The Bioelectrochemical Society

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008113

Organization unit

Faculty of Science

Keywords in English

Glutathion; phytochelatins; reduced -SH; oxidized -S-S-; voltammetric methods; tributhylphosphine

Abstract

V originále

Glutathione (L-g-glutamyl-L-cysteinyl-glycine, GSH) occurs in different species of animals, plants, and procaryotes. It is a starting material for the synthesis of some peptides and proteins such as phytochelatins (PCs). Glutathione and phytochelatins play a key role in the detoxication of heavy metals in plant cells. In addition, GSH is a powerful antioxidant. Electrochemical methods have been employed in analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cystein residues (reduced -SH/oxidized -S-S-). We used voltammetric methods in both differential pulse and square wave modes to study GSH and PCs redox states on a hanging mercury drop electrode. If the oxidation of reduced forms of the peptides (GSH and PCs) was started at -0.8 V (vs. Ag/AgCl/3M KCl), two reversible anodic peaks at -0.45 and -0.30 V were obtained. The first peak corresponds to the oxidation of the sulfhydryl group and its height is proportional to the amount of oxidized glutathione (GSSG) and PCs in a solution. Anodic signals were observed after aeration and/or addition of hydrogen peroxide to a solution containing reduced forms of peptides. The reducing agent tributyl phosphine showed the ability to preserve the reduction state of peptides. The study of the redox state of GSH and PCs is of great significance for the determination of redox cellular functions and the application of tributyl phosphine to preservation of reduced peptides is important in bioelectrochemical technology.

Links

GA203/02/0422, research and development project

Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

IAA1163201, research and development project

Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

Citovat

VACEK, Jan, Libuše TRNKOVÁ, René KIZEK and František JELEN. Electrochemical determination of glutathion and phytochelatins redox state. In XVII th International Symposium on Bioelectrochemistry and Bioenergetics. 1st ed. Florence, Italy: The Bioelectrochemical Society, 2003, p. 251.

@inproceedings{487726,
   author = {Vacek, Jan and Trnková, Libuše and Kizek, René and Jelen, František},
   address = {Florence, Italy},
   booktitle = {XVII th International Symposium on Bioelectrochemistry and Bioenergetics},
   edition = {1},
   keywords = {Glutathion; phytochelatins; reduced -SH; oxidized -S-S-; voltammetric methods; tributhylphosphine},
   language = {eng},
   location = {Florence, Italy},
   pages = {251-251},
   publisher = {The Bioelectrochemical Society},
   title = {Electrochemical determination of glutathion and phytochelatins redox state},
   year = {2003}
}

TY  - JOUR
ID  - 487726
AU  - Vacek, Jan - Trnková, Libuše - Kizek, René - Jelen, František
PY  - 2003
TI  - Electrochemical determination of glutathion and phytochelatins redox state
PB  - The Bioelectrochemical Society
CY  - Florence, Italy
KW  - Glutathion
KW  - phytochelatins
KW  - reduced -SH
KW  - oxidized -S-S-
KW  - voltammetric methods
KW  - tributhylphosphine
N2  - Glutathione (L-g-glutamyl-L-cysteinyl-glycine, GSH) occurs in different species of animals, plants, and procaryotes. It is a starting material for the synthesis of some peptides and proteins such as phytochelatins (PCs). Glutathione and phytochelatins play a key role in the detoxication of heavy metals in plant cells. In addition, GSH is a powerful antioxidant. Electrochemical methods have been employed in analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cystein residues (reduced -SH/oxidized -S-S-). We used voltammetric methods in both differential pulse and square wave modes to study GSH and PCs redox states on a hanging mercury drop electrode. If the oxidation of reduced forms of the peptides (GSH and PCs) was started at -0.8 V (vs. Ag/AgCl/3M KCl), two reversible anodic peaks at -0.45 and -0.30 V were obtained. The first peak corresponds to the oxidation of the sulfhydryl group and its height is proportional to the amount of oxidized glutathione (GSSG) and PCs in a solution. Anodic signals were observed after aeration and/or addition of hydrogen peroxide to a solution containing reduced forms of peptides. The reducing agent tributyl phosphine showed the ability to preserve the reduction state of peptides. The study of the redox state of GSH and PCs is of great significance for the determination of redox cellular functions and the application of tributyl phosphine to preservation of reduced peptides is important in bioelectrochemical technology.
ER  -

VACEK, Jan, Libuše TRNKOVÁ, René KIZEK and František JELEN. Electrochemical determination of glutathion and phytochelatins redox state. In \textit{XVII th International Symposium on Bioelectrochemistry and Bioenergetics}. 1st ed. Florence, Italy: The Bioelectrochemical Society, 2003, p.~251.

Detailed Information on Publication Record