Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium

MATIASOVIČOVÁ, Jitka, Marcela FALDYNOVÁ, Martina PRAVCOVÁ, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ, Jiří DAMBORSKÝ and Ivan RYCHLÍK. Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters. 2003, vol. 223, No 2, p. 281-286. ISSN 0378-1097.

Basic information

• Original name: Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium
• Authors: MATIASOVIČOVÁ, Jitka (203 Czech Republic), Marcela FALDYNOVÁ (203 Czech Republic), Martina PRAVCOVÁ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic), Ivana KOLÁČKOVÁ (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic, guarantor) and Ivan RYCHLÍK (203 Czech Republic).
• Edition: FEMS Microbiology Letters, 2003, 0378-1097.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.932
• RIV identification code: RIV/00216224:14310/03:00008817
• Organization unit: Faculty of Science
• UT WoS: 000183964700021
• Keywords in English: RRTT; TYPHYMURIUM; SALMONELLA; ENTERICA
• Tags: ENTERICA, RRTT, Salmonella, TYPHYMURIUM

Abstract

Bacterial retron reverse transcriptases are unusual enzymes which utilise the same RNA molecule as a template and also as a primer for initiation of the reverse transcription. Except for their relatively frequent presence in Myxococcus spp., they are considered as quite rare proteins. However, in this study we proved that retron reverse transcriptase is frequently found in certain serovars of Salmonella enterica. Using PCR, in strains of serovar Typhimurium, the rrtT (retron reverse transcriptase Typhimurium) gene was detected in 158 out of 175 tested field strains. On the other hand, in none of the 18 tested serovar Enteritidis strains the rrtT was detected in their genome. Detailed computer analysis allowed us to predict the sequence of msDNA and to propose that the final msDNA is free of any RNA. Furthermore, we predict that there are at least three different classes of retron reverse transcriptases.

Links

• LN00A016, research and development project: Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center