D 2003

Hydrophilic particles designed for PCR applications

ŠPANOVÁ, Alena, Eva SOUDKOVÁ, Bohuslav RITTICH and Daniel HORÁK

Basic information

Original name

Hydrophilic particles designed for PCR applications

Authors

ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Eva SOUDKOVÁ (203 Czech Republic), Bohuslav RITTICH (203 Czech Republic) and Daniel HORÁK (203 Czech Republic)

Edition

Moskva, 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography, p. 127-127, 2003

Publisher

Russian Academy of Sciences

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Russian Federation

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/03:00008119

Organization unit

Faculty of Science

Keywords in English

magnetic microspheres; magnetite; PCR; inhibition
Změněno: 15/7/2003 12:42, doc. Ing. Bohuslav Rittich, CSc.

Abstract

V originále

Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interferingmagnetic microspheres.

Links

GA202/02/1361, research and development project
Name: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice
Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics
QD1377, research and development project
Name: Vývoj metod hodnocení a rychlé identifikace vybraných bakterií mléčného kvašení s využitím metod molekulární biologie
Investor: Ministry of Agriculture of the CR, Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods