ŠPANOVÁ, Alena, Eva SOUDKOVÁ, Bohuslav RITTICH and Daniel HORÁK. Hydrophilic particles designed for PCR applications. In 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography. Moskva: Russian Academy of Sciences, 2003, p. 127.
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Basic information
Original name Hydrophilic particles designed for PCR applications
Authors ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Eva SOUDKOVÁ (203 Czech Republic), Bohuslav RITTICH (203 Czech Republic) and Daniel HORÁK (203 Czech Republic).
Edition Moskva, 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography, p. 127-127, 2003.
Publisher Russian Academy of Sciences
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Russian Federation
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/03:00008119
Organization unit Faculty of Science
Keywords in English magnetic microspheres; magnetite; PCR; inhibition
Tags inhibition, Magnetic microspheres, magnetite, PCR
Changed by Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 15/7/2003 12:42.
Abstract
Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interferingmagnetic microspheres.
Links
GA202/02/1361, research and development projectName: Využití magnetických nosičů s imobilizovanými selektivními ligandy v DNA diagnostice
Investor: Czech Science Foundation, The use of magnetic carriers with immobilized selective ligands in DNA diagnostics
QD1377, research and development projectName: Vývoj metod hodnocení a rychlé identifikace vybraných bakterií mléčného kvašení s využitím metod molekulární biologie
Investor: Ministry of Agriculture of the CR, Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods
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